Recombinant人PDPK1 protein (ab60834)

概述

描述

  • 性质
    Recombinant
  • 来源
    Baculovirus infected Sf9 cells
  • 氨基酸序列
    • 种属
      Human

技术指标

Our Abpromise guarantee covers the use of ab60834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性
    Specific Activity: 123 nmol/min/mg.
  • 应用

    Functional Studies

    SDS-PAGE

    Western blot

  • 形式
    Liquid
  • 补充说明

    ab204873 (AKT1 + PKN2 peptide substrate) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: 150mM Imidazole
    Constituents: 25% Glycerol, 50mM Sodium phosphate, 300mM Sodium chloride, 0.2mM DTT, 0.1mM PMSF, pH 7.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • 3 phosphoinositide dependent protein kinase 1
    • 3-phosphoinositide-dependent protein kinase 1
    • hPDK 1
    • hPDK1
    • MGC20087
    • MGC35290
    • OTTHUMP00000159109
    • OTTHUMP00000159110
    • OTTHUMP00000174525
    • PDK1
    • Pdpk1
    • PDPK1_HUMAN
    • PDPK2
    • PDPK2P
    • PkB kinase
    • PkB kinase like gene 1
    • PkB like 1
    • PRO0461
    • Protein kinase
    see all
  • 功能
    Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
  • 组织特异性
    Appears to be expressed ubiquitously.
  • 序列相似性
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
  • 细胞定位
    Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
  • Information by UniProt

图片

  • Sample Kinase Activity Plot.
  • ab60834 on SDS-PAGE, MW ~67kDa.
  • Anti-PDPK1 antibody (ab31406) at 1/1000 dilution + Recombinant human PDPK1 protein (ab60834) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

文献

ab60834 has not yet been referenced specifically in any publications.

客户评价及客户问答

There are currently no Customer reviews or Questions for ab60834.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册