Recombinant人PDP2 protein (ab110358)

概述

  • 产品名称Recombinant人PDP2 protein
  • 蛋白长度Full length protein

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionQ9P2J9
    • 种属Human
    • 氨基酸67 to 529
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab110358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Sandwich ELISA

    Functional Studies

  • 纯度> 90 % Purified via His tag.

  • 形式Liquid
  • 补充说明

     

    PDH Kinases (PDKs) deactivate PDH by reversible phosphorylation. In humans and rodents, 4 PDK's are present in an organ-dependent manner. In all cases, PDK's are physically part of the PDH complex (binding via lipoyl domain 2 of E2), and they phosphorylate the E1 subunit at 3 specific serine residues. PDH phosphatases remove the phosphorylation and restore activity of PDH.

    Product was previously marketed under the MitoSciences sub-brand.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.14% Sodium phosphate, 1.46% Sodium chloride, 30% Glycerol, 0.08% DTT, 0.02% EDTA, 0.05% Triton-X-100

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 2
    • KIAA1348
    • mitochondrial
    • PDP 2
    • Pdp2
    • PDP2_HUMAN
    • PDPC 2
    • Pyruvate dehydrogenase phosphatase 2
    • Pyruvate dehydrogenase phosphatase catalytic subunit 2
    • pyruvate dehydrogenase phosphatase isoenzyme 2
    • Pyruvate dehydrogenase phosphatase, catalytic subunit 2
    see all
  • 功能Catalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex.
  • 序列相似性Belongs to the PP2C family.
  • 细胞定位Mitochondrion matrix.
  • Information by UniProt

Recombinant human PDP2 protein (ab110358)参考文献

ab110358 has not yet been referenced specifically in any publications.

Product Wall

We advise use of PBS for homogenization of muscle tissue. We have not tested the use of this kit with fat tissue. If you have a favorite homogenization buffer, we would advice to prepare the homogenates of both tissues in both buffers in parallel , run...

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Thank you for your enquiry.

I can confirm that these proteins have been used in functional assays in the following publications using Pyruvate-dehydrogenase-PDH-Enzyme-Activity-Microplate-Assay-Kit-ab109902:

http://www.abcam.com/i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"