Recombinant人PDK4 protein (ab110356)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionQ16654
    • 种属Human
    • 氨基酸11 to 411
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab110356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性PDK4 activity was measured in a serial dilution of the recombinant protein and the minimum amount of the protein required to phosphorylate and fully inhibit PDH immunocaptured from given amount of human, bovine, rat or mouse samples was determined.
  • 应用

    Functional Studies

    Sandwich ELISA

  • 纯度> 90 % Purified via His tag.
    PDK4 was purified by metal chelation column chromatography (Ni-IDA) using His Binding Columns.
  • 形式Liquid
  • 补充说明

     

    PDH Kinases (PDKs) deactivate PDH by reversible phosphorylation. In humans and rodents, 4 PDK's are present in an organ-dependent manner. In all cases, PDK's are physically part of the PDH complex (binding via lipoyl domain 2 of E2), and they phosphorylate the E1 subunit at 3 specific serine residues. PDH phosphatases remove the phosphorylation and restore activity of PDH.

    Product was previously marketed under the MitoSciences sub-brand.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.14% Sodium phosphate, 1.46% Sodium chloride, 30% Glycerol, 0.08% DTT, 0.02% EDTA, 0.05% Triton-X-100

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4
    • FLJ40832
    • mitochondrial
    • Pdk4
    • PDK4_HUMAN
    • Pyruvate dehydrogenase [lipoamide] kinase isozyme 4 mitochondrial
    • Pyruvate dehydrogenase kinase 4
    • Pyruvate dehydrogenase kinase isoenzyme 4
    • Pyruvate dehydrogenase kinase isoform 4
    • Pyruvate dehydrogenase kinase isozyme 4
    • Pyruvate dehydrogenase kinase isozyme 4 mitochondrial
    see all
  • 功能Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
  • 组织特异性Ubiquitous; highest levels of expression in heart and skeletal muscle.
  • 序列相似性Belongs to the PDK/BCKDK protein kinase family.
    Contains 1 histidine kinase domain.
  • 细胞定位Mitochondrion matrix.
  • Information by UniProt

Recombinant human PDK4 protein (ab110356)参考文献

ab110356 has not yet been referenced specifically in any publications.

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