Recombinant人PDGF AB protein (ab73228)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 序列The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Ile-Glu-Glu- (A chain) and Met-Ser-Leu-Gly-Ser (B chain).

技术指标

Our Abpromise guarantee covers the use of ab73228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Specific Activity of 1 MIU/mg.
  • 应用

    SDS-PAGE

    Functional Studies

  • 纯度> 95 % SDS-PAGE.
    This protein was purified by proprietary chromatographic techniques. Purity greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
  • 形式Lyophilised
  • 补充说明For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    No additives

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶It is recommended to reconstitute the lyophilized PDGF AB in sterile 18Mohm/cm H2O at not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

常规信息

  • 别名
    • becaplermin
    • c-sis
    • GDGF
    • ODGF
    • Oncogene SIS
    • PDGF A chain
    • PDGF B chain
    • PDGF subunit A
    • PDGF subunit B
    • PDGF1
    • PDGF2
    • PDGFA
    • PDGFB
    • platelet-derived growth factor 2
    • platelet-derived growth factor A chain
    • platelet-derived growth factor alpha chain
    • platelet-derived growth factor alpha isoform 2 preproprotein
    • platelet-derived growth factor alpha polypeptide
    • platelet-derived growth factor B chain
    • platelet-derived growth factor beta polypeptide
    • platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)
    • platelet-derived growth factor subunit A
    • platelet-derived growth factor subunit B
    • platelet-derived growth factor, beta polypeptide (oncogene SIS)
    • proto-oncogene c-Sis
    • Simian sarcoma viral oncogene homolog
    • SIS
    • SSV
    • V-SIS platelet-derived growth factor beta polypeptide
    see all
  • 相关性Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains joined by disulphide bonds, termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Human PDGF-AB is a 25.5 kDa heterodimer protein consisting of a 13.3 kDa A chain and a 12.2 kDa B chain.
  • 细胞定位Secreted

Recombinant human PDGF AB protein (ab73228)参考文献

ab73228 has not yet been referenced specifically in any publications.

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