The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. Greater than 98% by SDS-PAGE and HPLC analyses.
Endotoxin level is <0.1 ng per µg.
The lyophilized protein is best-stored desiccated below 0oC. Reconstituted human PDGF-AB should be stored in working aliquots at -20oC.
This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or –20oC for future use.
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 10mM Acetic acid
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Reconstitute in 10 mM acetic acid to a concentration of 0.1-1.0 mg/ml.
Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains joined by disulphide bonds, termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Human PDGF-AB is a 25.5 kDa heterodimer protein consisting of a 13.3 kDa A chain and a 12.2 kDa B chain.
Recombinant human PDGF AB protein (ab52112)参考文献
has not yet been referenced specifically in any publications.