重组人PDGF AA蛋白(Animal Free) (ab179505)
Key features and details
- Expression system: Escherichia coli
- Purity: > 97% SDS-PAGE
- Endotoxin level: <= 1.000 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组人PDGF AA蛋白(Animal Free)
参阅全部 PDGF AA 蛋白酶 -
生物活性
The activity is determined by the dose-dependent proliferation of mouse 3T3 indicator cells and is typically 3-5 ng/mL.
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纯度
> 97 % SDS-PAGE. -
内毒素水平
<=1.000 Eu/µg -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
Yes -
性质
Recombinant -
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种属
Human -
序列
SIEEAVPAVC KTRTVIYEIP RSQVDPTSAN FLIWPPCVEV KRCTGCCNTS SVKCQPSRVH HRSVKVAKVE YVRKKPKLKE VQVRLEEHLE CACATTSLNP DYREEDTGRP RESGKKRKRK RLKPT -
预测分子量
29 kDa -
氨基酸
87 to 211 -
额外的序列信息
ab179505 is a non-glycosylated disulfide-linked homodimer, containing two 125 amino acid chains.
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技术指标
Our Abpromise guarantee covers the use of ab179505 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at -20°C long term. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions.
常规信息
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别名
- PDGF A
- PDGF A chain
- PDGF subunit A
see all -
功能
Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound. -
序列相似性
Belongs to the PDGF/VEGF growth factor family. -
结构域
The long form contains a basic insert which acts as a cell retention signal. -
细胞定位
Secreted. - Information by UniProt
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab179505 尚未被引用在任何文献中。