Recombinant人PDE9A protein (ab54113)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • 种属Human
    • 标签GST tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab54113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Specific Activity: 97 U/ug. 1 unit will release 1 pmole phosphate per minute from 400 uM 5’-GMP at 30 °C in a reaction buffer of 10 mM Tris-HCl, pH 7.4, 1 mM MgCl2. Assay buffer: 10mM Tris-HCl, pH 7.4, 1 mM Magnesium chloride ; 3’, 5’-cGMP substrate: 0.4 mM; PDE9A: 5 ng/ul.
  • 应用

    SDS-PAGE

    Phosphatase Activity

    Inhibition Assay

  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 30% Glycerol, 0.05% Tween 20, 5mM Glutathione, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • 5''-cyclic phosphodiesterase 9A
    • cGMP specific 3' 5' cyclic phosphodiesterase type 9
    • FLJ90181
    • High affinity cGMP-specific 3''
    • High-affinity cGMP-specific 3'5'-cyclic phosphodiesterase 9A
    • HSPDE9A2
    • OTTHUMP00000109399
    • PDE 9A
    • Pde9a
    • PDE9A_HUMAN
    • Phosphodiesterase 9A
    • phosphodiesterase PDE9A21
    see all
  • 功能Hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes.
  • 组织特异性Expressed in all tissues examined (testis, brain, small intestine, skeletal muscle, heart, lung, thymus, spleen, placenta, kidney, liver, pancreas, ovary and prostate) except blood. Highest levels in brain, heart, kidney, spleen, prostate and colon. Isoform PDE9A12 is found in prostate.
  • 序列相似性Belongs to the cyclic nucleotide phosphodiesterase family. PDE9 subfamily.
  • 结构域Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.
  • 细胞定位Cytoplasm. Endoplasmic reticulum; Cell projection > ruffle membrane. Cytoplasm > perinuclear region and Cell projection > ruffle membrane. Cytoplasm > perinuclear region. Golgi apparatus. Endoplasmic reticulum.
  • Information by UniProt

Recombinant human PDE9A protein (ab54113)参考文献

ab54113 has not yet been referenced specifically in any publications.

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