The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
生物活性Specific Activity: 97 U/ug. 1 unit will release 1 pmole phosphate per minute from 400 uM 5’-GMP at 30 °C in a reaction buffer of 10 mM Tris-HCl, pH 7.4, 1 mM MgCl2. Assay buffer: 10mM Tris-HCl, pH 7.4, 1 mM Magnesium chloride ; 3’, 5’-cGMP substrate: 0.4 mM; PDE9A: 5 ng/ul.
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Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
功能Hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes.
组织特异性Expressed in all tissues examined (testis, brain, small intestine, skeletal muscle, heart, lung, thymus, spleen, placenta, kidney, liver, pancreas, ovary and prostate) except blood. Highest levels in brain, heart, kidney, spleen, prostate and colon. Isoform PDE9A12 is found in prostate.
序列相似性Belongs to the cyclic nucleotide phosphodiesterase family. PDE9 subfamily.
结构域Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.