Recombinant人PDE9A protein (ab125666)

概述

描述

  • 性质
    Recombinant
  • 来源
    Baculovirus infected Sf9 cells
  • 氨基酸序列
    • Accession
    • 种属
      Human
    • 分子量
      86 kDa including tags
    • 氨基酸
      1 to 533

技术指标

Our Abpromise guarantee covers the use of ab125666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性
    The specific activity of ab125666 was determined to be 53 - 60 nmol/min/mg.
  • 应用

    Functional Studies

    SDS-PAGE

  • 纯度
    > 90 % Densitometry.
    Purity was determined to be >90% by densitometry.
  • 形式
    Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • 5''-cyclic phosphodiesterase 9A
    • cGMP specific 3' 5' cyclic phosphodiesterase type 9
    • FLJ90181
    • High affinity cGMP-specific 3''
    • High-affinity cGMP-specific 3'5'-cyclic phosphodiesterase 9A
    • HSPDE9A2
    • OTTHUMP00000109399
    • PDE 9A
    • Pde9a
    • PDE9A_HUMAN
    • Phosphodiesterase 9A
    • phosphodiesterase PDE9A21
    see all
  • 功能
    Hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes.
  • 组织特异性
    Expressed in all tissues examined (testis, brain, small intestine, skeletal muscle, heart, lung, thymus, spleen, placenta, kidney, liver, pancreas, ovary and prostate) except blood. Highest levels in brain, heart, kidney, spleen, prostate and colon. Isoform PDE9A12 is found in prostate.
  • 序列相似性
    Belongs to the cyclic nucleotide phosphodiesterase family. PDE9 subfamily.
  • 结构域
    Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.
  • 细胞定位
    Cytoplasm. Endoplasmic reticulum; Cell projection > ruffle membrane. Cytoplasm > perinuclear region and Cell projection > ruffle membrane. Cytoplasm > perinuclear region. Golgi apparatus. Endoplasmic reticulum.
  • Information by UniProt

图片

  • SDS-PAGE analysis of ab125666.
  • Sample Phosphodiesterase Assay showing the specific activity of ab125666 to be 60 nmol/min/mg.

文献

ab125666 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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