重组人PDE9A蛋白(ab125666)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组人PDE9A蛋白
参阅全部 PDE9A 蛋白酶 -
生物活性
The specific activity of ab125666 was determined to be 53 - 60 nmol/min/mg.
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纯度
> 90 % Densitometry.
Purity was determined to be >90% by densitometry. Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
86 kDa including tags -
氨基酸
1 to 533
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技术指标
Our Abpromise guarantee covers the use of ab125666 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- 5''-cyclic phosphodiesterase 9A
- cGMP specific 3' 5' cyclic phosphodiesterase type 9
- FLJ90181
see all -
功能
Hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes. -
组织特异性
Expressed in all tissues examined (testis, brain, small intestine, skeletal muscle, heart, lung, thymus, spleen, placenta, kidney, liver, pancreas, ovary and prostate) except blood. Highest levels in brain, heart, kidney, spleen, prostate and colon. Isoform PDE9A12 is found in prostate. -
序列相似性
Belongs to the cyclic nucleotide phosphodiesterase family. PDE9 subfamily. -
结构域
Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain. -
细胞定位
Cytoplasm. Endoplasmic reticulum; Cell projection > ruffle membrane. Cytoplasm > perinuclear region and Cell projection > ruffle membrane. Cytoplasm > perinuclear region. Golgi apparatus. Endoplasmic reticulum. - Information by UniProt
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab125666 尚未被引用在任何文献中。