Recombinant人PDE4A protein (ab125603)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • AccessionP27815
    • 种属Human
    • 分子量110 kDa including tags
    • 氨基酸332 to 886
    • 标签GST tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab125603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性The specific activity was determined to be 1082 nmol/min/mg
  • 应用

    Functional Studies

    SDS-PAGE

  • 纯度> 95 % Densitometry.

  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • 5''-cyclic phosphodiesterase 4A
    • cAMP specific phosphodiesterase
    • cAMP-specific 3''
    • cAMP-specific 3',5'-cyclic phosphodiesterase 4A
    • DPDE2
    • dunce like phosphodiesterase E2
    • PDE 4A
    • PDE4
    • PDE46
    • PDE4A
    • PDE4A_HUMAN
    • PDE4A11
    • Phosphodiesterase 4A
    • phosphodiesterase 4A cAMP specific
    • Phosphodiesterase 4A, cAMP-specific (dunce
    • phosphodiesterase E2 dunce homolog
    • Phosphodiesterase E2 dunce homolog, Drosophila
    • phosphodiesterase isozyme 4
    • phosphodiesterase type 4A11
    see all
  • 功能Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
  • 组织特异性Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum (at protein level).
  • 通路Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
  • 序列相似性Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
  • 翻译后修饰Phosphorylated at Ser-686 and Ser-688 when expressed in S.frugiperda cells. Isoform 2 and isoform 7 are activated by phosphorylation at Ser-119 and Ser-123 respectively by PKA.
    Proteolytically cleaved by caspase-3.
  • 细胞定位Cytoplasm > perinuclear region; Cytoplasm > perinuclear region. Cell projection > ruffle membrane; Cytoplasm. Membrane. Predominantly cytoplasmic and Membrane. Isoform 4 has propensity for association with membranes.
  • Information by UniProt

Recombinant human PDE4A protein 图像

Recombinant human PDE4A protein (ab125603)参考文献

ab125603 has not yet been referenced specifically in any publications.

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