Recombinant人PDE4A protein (ab117037)

概述

描述

  • 性质Recombinant
  • 来源Wheat germ
  • 氨基酸序列
    • AccessionP27815-4
    • 种属Human
    • 序列MPLVDFFCETCSKPWLVGWWDQFKRMLNRELTHLSEMSRSGNQVSEYIST TFLDKQNEVEIPSPTMKEREKQQAPRPRPSQPPPPPVPHLQPMSQITGLK KLMHSNSLNNSNIPRFGVKTDQEELLAQELENLNKWGLNIFCVSDYAGGR SLTCIMYMIFQERDLLKKFRIPVDTMVTYMLTLEDHYHADVAYHNSLHAA DVLQSTHVLLATPALDAVFTDLEILAALFAAAIHDVDHPGVSNQFLINTN SELALMYNDESVLENHHLAVGFKLLQEDNCDIFQNLSKRQRQSLRKMVID MVLATDMSKHMTLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLRNMVHC ADLSNPTKPLELYRQWTDRIMAEFFQQGDRERERGMEISPMCDKHTASVE KSQVGFIDYIVHPLWETWADLVHPDAQEILDTLEDNRDWYYSAIRQSPSP PPEEESRGPGHPPLPDKFQFELTLEEEEEEEISMAQIPCTAQEALTAQGL SGVEEALDATIAWEASPAQESLEVMAQEASLEAELEAVYLTQQAQSTGSA PVAPDEFSSREEFVVAVSHSSPSALALQSPLLPAWRTLSVSEHAPGLPGL PSTAAEVEAQREHQAAKRACSACAGTFGEDTSALPAPGGGGSGGDPT
    • 分子量97 kDa including tags
    • 氨基酸1 to 647
    • 标签GST tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab117037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    ELISA

    SDS-PAGE

    Western blot

  • 形式Liquid
  • 补充说明Protein concentration is above or equal to 0.05 mg/ml.
    Best use within three months from the date of receipt of this protein.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

常规信息

  • 别名
    • 5''-cyclic phosphodiesterase 4A
    • cAMP specific phosphodiesterase
    • cAMP-specific 3''
    • cAMP-specific 3',5'-cyclic phosphodiesterase 4A
    • DPDE2
    • dunce like phosphodiesterase E2
    • PDE 4A
    • PDE4
    • PDE46
    • PDE4A
    • PDE4A_HUMAN
    • PDE4A11
    • Phosphodiesterase 4A
    • phosphodiesterase 4A cAMP specific
    • Phosphodiesterase 4A, cAMP-specific (dunce
    • phosphodiesterase E2 dunce homolog
    • Phosphodiesterase E2 dunce homolog, Drosophila
    • phosphodiesterase isozyme 4
    • phosphodiesterase type 4A11
    see all
  • 功能Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
  • 组织特异性Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum (at protein level).
  • 通路Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
  • 序列相似性Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
  • 翻译后修饰Phosphorylated at Ser-686 and Ser-688 when expressed in S.frugiperda cells. Isoform 2 and isoform 7 are activated by phosphorylation at Ser-119 and Ser-123 respectively by PKA.
    Proteolytically cleaved by caspase-3.
  • 细胞定位Cytoplasm > perinuclear region; Cytoplasm > perinuclear region. Cell projection > ruffle membrane; Cytoplasm. Membrane. Predominantly cytoplasmic and Membrane. Isoform 4 has propensity for association with membranes.
  • Information by UniProt

Recombinant Human PDE4A protein 图像

  • 12.5% SDS-PAGE stained with Coomassie Blue showing ab117037 at approximately 97.24 kDa.

Recombinant Human PDE4A protein (ab117037)参考文献

ab117037 has not yet been referenced specifically in any publications.

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