重组人PD1蛋白(Active) (ab221328)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: StrepII tag C-Terminus
- Suitable for: SDS-PAGE, ELISA
描述
-
产品名称
重组人PD1蛋白(Active)
参阅全部 PD1 蛋白酶 -
生物活性
Measured by its binding ability in a functional ELISA. Immobilized ab221328 at 2 µg/mL (100 µL/well) can bind Human PD-L2, Fc Tag with a linear range of 0.4-6 ng/mL.
Measured by its binding ability in a functional ELISA. Immobilized ab221328 at 2 µg/mL (100 µL/well) can bind Human PD-L1, Fc Tag with a linear range of 4-63 ng/mL.
Measured by its binding ability in a functional ELISA. Immobilized ab221328 at 2 µg/mL (100 µL/well) can bind Nivolumab with a linear range of 0.1-3 ng/mL.
-
纯度
> 90 % SDS-PAGE. -
内毒素水平
< 1.000 Eu/µg -
表达系统
HEK 293 cells -
Accession
-
蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
-
种属
Human -
序列
LDSPDR PWNPPTFSPA LLVVTEGDNA TFTCSFSNTS ESFVLNWYRM SPSNQTDKLA AFPEDRSQPG QDCRFRVTQL PNGRDFHMSV VRARRNDSGT YLCGAISLAP KAQIKESLRA ELRVTERRAE VPTAHPSPSP RPAGQFQ -
预测分子量
20 kDa including tags -
氨基酸
25 to 167 -
标签
StrepII tag C-Terminus -
额外的序列信息
(NP_005009.2).
-
相关产品
-
Related Products
技术指标
Our Abpromise guarantee covers the use of ab221328 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
应用
SDS-PAGE
ELISA
-
形式
Lyophilized -
补充说明
Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
-
Concentration information loading...
制备和贮存
-
稳定性和存储
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 95% PBS, 5% Trehalose
Lyophilized from 0.22 µm filtered solution.This product is an active protein and may elicit a biological response in vivo, handle with caution.
-
复溶Reconstitute with sterile deionized water to a concentration of 400 µg/ml.
常规信息
-
别名
- CD279
- CD279 antigen
- hPD 1
see all -
功能
Possible cell death inducer, in association with other factors. -
疾病相关
Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
序列相似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
发展阶段
Induced at programmed cell death. -
细胞定位
Membrane. - Information by UniProt
图片
-
SDS-PAGE of reduced ab221328 stained overnight with Coomassie Blue. The protein migrates as 30-45 kDa due to glycosylation.
-
Immobilized ab221328 at 2 µg/mL (100 µL/well) can bind Nivolumab with a linear range of 0.1-3 ng/mL.
-
Immobilized ab221328 at 2 µg/mL (100 µL/well) can bind Human PD-L1, Fc Tag with a linear range of 4-63 ng/mL.
-
Immobilized ab221328 at 2 µg/mL (100 µL/well) can bind Human PD-L2, Fc Tag with a linear range of 0.4-6 ng/mL.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
Datasheet download
文献 (0)
ab221328 尚未被引用在任何文献中。