Recombinant人PCK1 protein (ab119469)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionP35558
    • 种属Human
    • 序列MGSSHHHHHH SSGLVPRGSH MGSHMPPQLQ NGLNLSAKVV QGSLDSLPQA VREFLENNAE LCQPDHIHIC DGSEEENGRL LGQMEEEGIL RRLKKYDNCW LALTDPRDVA RIESKTVIVT QEQRDTVPIP KTGLSQLGRW MSEEDFEKAF NARFPGCMKG RTMYVIPFSM GPLGSPLSKI GIELTDSPYV VASMRIMTRM GTPVLEALGD GEFVKCLHSV GCPLPLQKPL VNNWPCNPEL TLIAHLPDRR EIISFGSGYG GNSLLGKKCF ALRMASRLAK EEGWLAEHML VLGITNPEGE KKYLAAAFPS ACGKTNLAMM NPSLPGWKVE CVGDDIAWMK FDAQGHLRAI NPENGFFGVA PGTSVKTNPN AIKTIQKNTI FTNVAETSDG GVYWEGIDEP LASGVTITSW KNKEWSSEDG EPCAHPNSRF CTPASQCPII DAAWESPEGV PIEGIIFGGR RPAGVPLVYE ALSWQHGVFV GAAMRSEATA AAEHKGKIIM HDPFAMRPFF GYNFGKYLAH WLSMAQHPAA KLPKIFHVNW FRKDKEGKFL WPGFGENSRV LEWMFNRIDG KASTKLTPIG YIPKEDALNL KGLGHINMME LFSISKEFWE KEVEDIEKYL EDQVNADLPC EIEREILALK QRISQM
    • 分子量72 kDa including tags
    • 氨基酸1 to 622
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab119469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

  • 纯度> 90 % SDS-PAGE.
    ab119469 was purified using conventional chromatography.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 10% Glycerol, 0.02% DTT, 0.58% Sodium chloride

常规信息

  • 别名
    • cytosolic [GTP]
    • GTP
    • PCK1
    • PCKGC_HUMAN
    • PEP carboxykinase
    • PEPCK-C
    • PEPCK1
    • PEPCKC
    • Phosphoenolpyruvate carboxykinase
    • Phosphoenolpyruvate carboxykinase 1
    • Phosphoenolpyruvate carboxykinase 1 (soluble)
    • Phosphoenolpyruvate carboxykinase, cytosolic
    • Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
    • Phosphoenolpyruvate carboxylase
    • Phosphopyruvate carboxylase
    see all
  • 功能Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
  • 组织特异性Major sites of expression are liver, kidney and adipocytes.
  • 通路Carbohydrate biosynthesis; gluconeogenesis.
  • 疾病相关Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (cytosolic PEPCK deficiency) [MIM:261680]. PEPCK deficiency is a metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait.
  • 序列相似性Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.
  • 翻译后修饰Acetylation is increased on addition of glucose and appears to regulate the protein stability.
  • 细胞定位Cytoplasm.
  • Information by UniProt

Recombinant Human PCK1 protein 图像

  • 15% SDS-PAGE analysis of ab119469 (3µg)

Recombinant Human PCK1 protein (ab119469)参考文献

ab119469 has not yet been referenced specifically in any publications.

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