Recombinant人PCK1 protein (ab114362)

概述

描述

  • 性质
    Recombinant
  • 来源
    Wheat germ
  • 氨基酸序列
    • Accession
    • 种属
      Human
    • 序列
      MPPQLQNGLNLSAKVVQGSLDSLPQAVREFLENNAELCQPDHIHICDGSE EENGRLLGQMEEEGILRRLKKYDNCWLALTDPRDVARIESKTVIVTQEQR DTVPIPKTGLSQLGRWMSEEDFEKAFNARFPGCMKGRTMYVIPFSMGPLG SPLSKIGIELTDSPYVVASMRIMTRMGTPVLEALGDGEFVKCLHSVGCPL PLQKPLVNNWPCNPELTLIAHLPDRREIISFGSGYGGNSLLGKKCFALRM ASRLAKEEGWLAEHMLVLGITNPEGEKKYLAAAFPSACGKTNLAMMNPSL PGWKVECVGDDIAWMKFDAQGHLRAINPENGFFGVAPGTSVKTNPNAIKT IQKNTIFTNVAETSDGGVYWEGIDEPLASGVTITSWKNKEWSSEDGEPCA HPNSRFCTPASQCPIIDAAWESPEGVPIEGIIFGGRRPAGVPLVYEALSW QHGVFVGAAMRSEATAAAEHKGKIIMHDPFAMRPFFGYNFGKYLAHWLSM AQHPAAKLPKIFHVNWFRKDKEGKFLWPGFGENSRVLEWMFNRIDGKAST KLTPIGYIPKEDALNLKGLGHINMMELFSISKEFWEKEVEDIEKYLEDQV NADLPCEIEREILALKQRISQM
    • 分子量
      95 kDa including tags
    • 氨基酸
      1 to 623

技术指标

Our Abpromise guarantee covers the use of ab114362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    ELISA

    SDS-PAGE

    Western blot

  • 形式
    Liquid
  • 补充说明
    Protein concentration is above or equal to 0.05 mg/ml. Best use within three months from the date of receipt of this protein.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

常规信息

  • 别名
    • cytosolic [GTP]
    • GTP
    • PCK1
    • PCKGC_HUMAN
    • PEP carboxykinase
    • PEPCK-C
    • PEPCK1
    • PEPCKC
    • Phosphoenolpyruvate carboxykinase
    • Phosphoenolpyruvate carboxykinase 1
    • Phosphoenolpyruvate carboxykinase 1 (soluble)
    • Phosphoenolpyruvate carboxykinase, cytosolic
    • Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
    • Phosphoenolpyruvate carboxylase
    • Phosphopyruvate carboxylase
    see all
  • 功能
    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
  • 组织特异性
    Major sites of expression are liver, kidney and adipocytes.
  • 通路
    Carbohydrate biosynthesis; gluconeogenesis.
  • 疾病相关
    Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (cytosolic PEPCK deficiency) [MIM:261680]. PEPCK deficiency is a metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait.
  • 序列相似性
    Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.
  • 翻译后修饰
    Acetylation is increased on addition of glucose and appears to regulate the protein stability.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt

图片

  • 12.5% SDS-PAGE showing ab114362 at approximately 94.53kDa stained with Coomassie Blue.

文献

ab114362 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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