Recombinant人PC4 (mutated S13 + S15 + S17 + S19) protein (ab81957)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 修饰mutated S

技术指标

Our Abpromise guarantee covers the use of ab81957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性1 unit equals 1 nanogram of purified protein.
  • 应用

    SDS-PAGE

    Functional Studies

    EMSA

  • 纯度> 95 % SDS-PAGE.
    ab81957 is purified by conventional chromatography.
  • 形式Liquid
  • 补充说明


    Wild-type PC4, but not the kinase inhibitory-deficient mutant of PC4 (serine mutations), represses specific transcription in vivo.

    1 unit equals 1 nanogram of purified protein.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 20mM Tris HCl, 100mM Potassium chloride, 1mM DTT, 0.2mM EDTA, pH 8.0

常规信息

  • 别名
    • Activated RNA polymerase II transcriptional coactivator p15
    • Interferon related developmental regulator 1
    • MGC102747
    • p14
    • P15
    • PC4
    • PC4 LSB
    • Positive cofactor 4
    • RPO2TC1
    • Sub1
    • SUB1 homolog
    • TCP4_HUMAN
    see all
  • 功能General coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA).
  • 序列相似性Belongs to the transcriptional coactivator PC4 family.
  • 翻译后修饰Activity is controlled by protein kinases that target the regulatory region. Phosphorylation inactivates both ds DNA-binding and cofactor function, but does not affect binding to ssDNA. Seems to be phosphorylated in vivo by CK2 in at least 7 sites in the N-terminal Ser-rich region.
  • 细胞定位Nucleus.
  • Information by UniProt

Recombinant Human PC4 (mutated S13 + S15 + S17 + S19) protein (ab81957)参考文献

ab81957 has not yet been referenced specifically in any publications.

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