功能Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
翻译后修饰Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR. Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites. S-nitrosylated, leading to inhibit transcription regulation activity.
Lane A: unmodified PARP
LANE B: automodified PARP
Lane B shows a smear of staining above MW 113kDa indicating polyADP-ribosylated PARP
modified to various extents. A significant amount
of automodified PARP did not enter the gel (top
This image shows that most of the PARP will be at the top of the gel with a smear running up from 113kDa indicating PARP is automodified to various extents. There is little or no native PARP at 113kDa.
Western blot - PARP protein (Automodified) (ab75605)
All lanes : anti-parp antibody at 5 µg/ml
Lane 1 : 100ng PARP- unmodified Lane 2 : 100ng ab75605 -automodified PARP