Recombinant人PARP protein (ab75605)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus
  • 氨基酸序列
    • 种属Human

技术指标

Our Abpromise guarantee covers the use of ab75605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot

    ELISA

    SDS-PAGE

  • 形式Lyophilised
  • 补充说明


    Reconstituted PARP will retain residual polyADP-ribosylation enzymatic activity.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents (upon reconstitution in 100ul distilled water): 50mM Tris, 100mM Sodium chloride, 10mM Magnesium chloride, 1mM DTT, 1mM NAD , 10 µg/mL activated DNA, plus lyophilization stabilizers. pH 8.

  • 复溶To reconstitute,add 100µl distilled water and leave for 5 min. Vortex gently for 1 min, avoiding air bubbles. Spin briefly.

常规信息

  • 别名
    • ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase)
    • ADP ribosyltransferase diphtheria toxin like 1
    • ADP ribosyltransferase NAD(+)
    • ADPRT
    • ADPRT 1
    • ADPRT1
    • ARTD1
    • msPARP
    • NAD(+) ADP ribosyltransferase 1
    • NAD(+) ADP-ribosyltransferase 1
    • pADPRT 1
    • pADPRT1
    • PARP
    • PARP 1
    • PARP-1
    • PARP1
    • PARP1_HUMAN
    • Poly (ADP ribose) polymerase 1
    • poly (ADP ribose) polymerase family, member 1
    • Poly [ADP-ribose] polymerase 1
    • Poly(ADP ribose) polymerase
    • poly(ADP ribose) synthetase
    • poly(ADP ribosyl)transferase
    • Poly[ADP ribose] synthetase 1
    • Poly[ADP-ribose] synthase 1
    • PPOL
    see all
  • 功能Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
  • 序列相似性Contains 1 BRCT domain.
    Contains 1 PARP alpha-helical domain.
    Contains 1 PARP catalytic domain.
    Contains 2 PARP-type zinc fingers.
  • 翻译后修饰Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
    S-nitrosylated, leading to inhibit transcription regulation activity.
  • 细胞定位Nucleus.
  • Information by UniProt

Recombinant Human PARP protein 图像

  • Lane A: unmodified PARP LANE B: automodified PARP Lane B shows a smear of staining above MW 113kDa indicating polyADP-ribosylated PARP modified to various extents. A significant amount of automodified PARP did not enter the gel (top arrow). This image shows that most of the PARP will be at the top of the gel with a smear running up from 113kDa indicating PARP is automodified to various extents. There is little or no native PARP at 113kDa.
  • All lanes : anti-parp antibody at 5 µg/ml

    Lane 1 : 100ng PARP- unmodified
    Lane 2 : 100ng ab75605 -automodified PARP


    Predicted band size : 113 kDa
    Observed band size : >250 kDa (why is the actual band size different from the predicted?)

Recombinant Human PARP protein (ab75605)参考文献

ab75605 has not yet been referenced specifically in any publications.

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