Recombinant人PARP protein (ab123834)



  • 性质Recombinant
  • 来源Baculovirus
  • 氨基酸序列
    • AccessionP09874
    • 种属Human
    • 分子量116 kDa
    • 氨基酸1 to 1014


Our Abpromise guarantee covers the use of ab123834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性1000 U/vial, specific activity = 20000 U/mg PARP1. 1U=10 fmol ADP-ribose incorporated into 5 µg immobilized histone in 30 min at room temperature. Note: Activity measurements are approximate values.
  • 应用

    Functional Studies


  • 纯度> 95 % SDS-PAGE.

  • 形式Lyophilised
  • 补充说明STORAGE CONDITIONS: Store reconstituted solution at -70°C. Avoid multiple freeze-thaw cycles. CAUTION: There is loss of PARP enzymatic activity upon each free/thaw cycle. It is suggested to aliquot the reconstituted enzyme into multiple tubes and freeze at -70°C. Alternatively, add glycerol at 1:1 vol/vol to the reconstituted PARP, mix gently by trituration, and store at -20°C (do not store in a frost-free freezer!) for up to 6 months.
  • Concentration information loading...


  • 稳定性和存储

    Shipped at 4°C. Store at -80°C.

    pH: 8.00
    Constituents: 0.02% DTT, 0.24% Tris, 0.003% EDTA, 1.74% Sodium chloride
    Note: Contains lyophilization stabilizers.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶Spin tube in a microfuge for 15 sec to sediment lyophilized material. Carefully open the vial and add 100 µL dH2O. Vortex gently for 20 sec (avoid air bubbles). Let stand for 5 min. Carefully triturate the sample 10-times using a pipetman (avoid air bubbles). Spin briefly in microfuge to consolidate.


  • 别名
    • ADP ribosyltransferase
    • ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase)
    • ADP ribosyltransferase diphtheria toxin like 1
    • ADP ribosyltransferase NAD(+)
    • ADPRT
    • ADPRT 1
    • ADPRT1
    • ARTD1
    • msPARP
    • NAD(+) ADP ribosyltransferase 1
    • NAD(+) ADP-ribosyltransferase 1
    • pADPRT 1
    • pADPRT1
    • PARP
    • PARP 1
    • PARP-1
    • PARP1
    • Poly (ADP ribose) polymerase 1
    • poly (ADP ribose) polymerase family, member 1
    • Poly [ADP-ribose] polymerase 1
    • Poly(ADP ribose) polymerase
    • poly(ADP ribose) synthetase
    • poly(ADP ribosyl)transferase
    • Poly[ADP ribose] synthetase 1
    • Poly[ADP-ribose] synthase 1
    • PPOL
    see all
  • 功能Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
  • 序列相似性Contains 1 BRCT domain.
    Contains 1 PARP alpha-helical domain.
    Contains 1 PARP catalytic domain.
    Contains 2 PARP-type zinc fingers.
  • 翻译后修饰Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
    S-nitrosylated, leading to inhibit transcription regulation activity.
  • 细胞定位Nucleus.
  • Information by UniProt

Recombinant human PARP protein (ab123834)参考文献

ab123834 has not yet been referenced specifically in any publications.

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