Recombinant人Parathyroid Hormone protein (ab51234)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 序列MSVSEIQLMH NLGKHLNSME RVEWLRKKLQ DVHNFVALGA PLAPRDAGSQ RPRKKEDNVL VESHEKSLGE ADKADVNVLT KAKSQ

技术指标

Our Abpromise guarantee covers the use of ab51234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.
    Human Parathyroid Hormone(amino acids 1-84) was overexpressed in E. coli and purified to apparent homogeneity by using conventional column chromatography techniques.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: PBS, 2mM EDTA, pH 7.4

常规信息

  • 别名
    • hPTH
    • Parathormone
    • Parathyrin
    • Parathyroid hormone
    • Parathyroid hormone 1
    • PTH
    • PTH1
    • PTH1 receptor
    • PTH1R
    • PTHR
    • PTHR1
    • PTHY_HUMAN
    see all
  • 功能PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
  • 疾病相关Defects in PTH are a cause of familial isolated hypoparathyroidism (FIH) [MIM:146200]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism.
  • 序列相似性Belongs to the parathyroid hormone family.
  • 细胞定位Secreted.
  • Information by UniProt

Recombinant Human Parathyroid Hormone protein 图像

  • Standard curve for Parathyroid Hormone (Analyte: ab51234); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [A1/70] to Parathyroid Hormone (ab14493) at 0.2µg/ml and Detector Antibody Rabbit polyclonal to Parathyroid Hormone (ab53040) at 0.5µg/ml.
  • ab51234 on 15%SDS-PAGE
  • Anti-Parathyroid Hormone antibody (ab40630) at 1 µg/ml + Recombinant Human Parathyroid Hormone protein (ab51234) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 4 minutes

Recombinant Human Parathyroid Hormone protein (ab51234)参考文献

ab51234 has not yet been referenced specifically in any publications.

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