The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
生物活性Specific Activity: 32638 pmoles/min/µg.
Analysis of enzymatic activity was performed according to Zlyte assay protocol:
Different concentration of PAK1 were incubated in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, and 2 µM substrate (SER/THR14) at room temperature for 1 hour.
Developer solution was added to reaction and reaction was stopped after 1h of incubation at RT.
Fluorescence was then detected using Lambda exc=460±40 nm and Lambda em=528±20 nm filters.
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Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
功能The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
序列相似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 CRIB domain. Contains 1 protein kinase domain.
翻译后修饰Autophosphorylated when activated by CDC42/p21 and RAC1.
细胞定位Cytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation.