Recombinant人PAK1 protein (ab67946)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • 种属Human

技术指标

Our Abpromise guarantee covers the use of ab67946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Functional Studies

    Western blot

  • 纯度> 90 % SDS-PAGE.

  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Constituents: 25% Glycerol, 50mM Sodium chloride, 50mM Tris HCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

常规信息

  • 别名
    • ADRB2
    • Alpha PAK
    • Alpha-PAK
    • MGC130000
    • MGC130001
    • p21 activated kinase 1
    • p21 protein (Cdc42/Rac) activated kinase 1
    • p21-activated kinase 1
    • p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related)
    • p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)
    • p65 PAK
    • p65-PAK
    • P68-PAK
    • PAK alpha
    • PAK-1
    • Pak1
    • PAK1_HUMAN
    • Paka
    • PAKalpha
    • Protein kinase MUK2
    • Rac/p21-activated kinase
    • Serine/threonine-protein kinase PAK 1
    • STE20 homolog yeast
    see all
  • 功能The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
  • 序列相似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CRIB domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated when activated by CDC42/p21 and RAC1.
  • 细胞定位Cytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation.
  • Information by UniProt

Recombinant Human PAK1 protein 图像

  • Anti-PAK1 antibody (ab1178) at 1/1000 dilution + Recombinant Human PAK1 protein (ab67946) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

Recombinant Human PAK1 protein (ab67946)参考文献

ab67946 has not yet been referenced specifically in any publications.

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