重组人PAI1 (mutated )蛋白(ab93232)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: Inhibition Assay, SDS-PAGE
描述
-
产品名称
重组人PAI1 (mutated )蛋白
参阅全部 PAI1 蛋白酶 -
生物活性
A triple mutation on the wild-type background makes ab93232 selectively inhibit tPA. Urokinase inhibition is limited to 1/25,000 of tPA inhibition by the P3Tyr-P2Ser-P1Tyr mutation. -
纯度
> 95 % SDS-PAGE. -
表达系统
Escherichia coli -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
-
种属
Human -
修饰
mutated P1Y + P2S + P3Y
-
技术指标
Our Abpromise guarantee covers the use of ab93232 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
应用
Inhibition Assay
SDS-PAGE
-
形式
Liquid -
补充说明
A triple mutation on the wild-type background makes ab93232 selectively inhibit tPA. Urokinase inhibition is limited to 1/25,000 of tPA inhibition by the P3Tyr-P2Ser-P1Tyr mutation. -
Concentration information loading...
制备和贮存
-
稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 6.60
Constituents: 0.82% Sodium phosphate, 0.0292% EDTA, 0.58% Sodium chloride
常规信息
-
别名
- Clade E
- Endothelial plasminogen activator inhibitor
- Nexin
see all -
功能
This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. -
组织特异性
Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells. -
疾病相关
Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions. -
序列相似性
Belongs to the serpin family. -
翻译后修饰
Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
-Met-370 bond. -
细胞定位
Secreted. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
Datasheet download
文献 (0)
ab93232 尚未被引用在任何文献中。