Recombinant人PAI1 (mutated N150 H + K154 T + Q319 L + M354 I) protein (ab92969)


  • 产品名称Recombinant人PAI1 (mutated N150 H + K154 T + Q319 L + M354 I) protein
    参阅全部 PAI1 蛋白酶
  • 蛋白长度Full length protein


  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human


Our Abpromise guarantee covers the use of ab92969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性>95% active by uPA titration. Kinetic Data: Second order rate constants for inhibition of: uPA = 5.1 X 106 M-1s-1 tPA = 7.9 X 105 M-1s-1
  • 应用


    Functional Studies

  • 纯度> 95 % SDS-PAGE.
    > 99% pure by SDS PAGE
  • 形式Liquid
  • 补充说明Solubility: > 2 mg/mL and < 5 mg/mL Ultraviolet: Absorbance (280nm) = 2.5 epsilon 0.1% = 1.0

    ab92969 contains the following four mutations: K154T, Q319L, M354I and N150H. These mutations combine to confer great stability to the otherwise labile molecule essentially locking in into the active conformation. It is an ideal choice for in vivo studies.

  • Concentration information loading...


  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50mM Sodium phosphate, 100mM Sodium chloride, 1mM EDTA, pH 6.6

    This product is an active protein and may elicit a biological response in vivo, handle with caution.


  • 别名
    • Clade E
    • Endothelial plasminogen activator inhibitor
    • Nexin
    • PAI
    • PAI 1
    • PAI-1
    • PAI1_HUMAN
    • PLANH1
    • Plasminogen activator inhibitor 1
    • Plasminogen activator inhibitor type 1
    • Serine (or cysteine) proteinase inhibitor
    • Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1
    • Serpin E1
    • Serpin peptidase inhibitor clade E
    • Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1
    • Serpine 1
    • SERPINE1
    see all
  • 功能This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
  • 组织特异性Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
  • 疾病相关Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
    Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
  • 序列相似性Belongs to the serpin family.
  • 翻译后修饰Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
    -Met-370 bond.
  • 细胞定位Secreted.
  • Information by UniProt

Recombinant human PAI1 (mutated N150 H + K154 T + Q319 L + M354 I) protein 图像

  • 10% SDS Page Gelcode Blue Stain
    1. 3ug ab92969 reduced
    2. 3ug ab92969 + 12ug Urokinase reduced
    3. prestained standard

Recombinant human PAI1 (mutated N150 H + K154 T + Q319 L + M354 I) protein (ab92969)参考文献

ab92969 has not yet been referenced specifically in any publications.

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