Recombinant人P4HB protein (ab115719)

概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • Accession
    • 种属
      Human
    • 分子量
      58 kDa
    • 标签
      His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab115719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性
    >200U/mg
  • 应用

    Western blot

    Functional Studies

    SDS-PAGE

  • 纯度
    > 95 % SDS-PAGE.
    ab115719 is purified by multi-step chromatography.
  • 形式
    Liquid
  • 补充说明
    >200U/mg
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.61% Tris, 0.88% Sodium chloride, 5% Glycerol, 0.02% DTT, 0.03% EDTA

常规信息

  • 别名
    • Cellular thyroid hormone binding protein
    • Cellular thyroid hormone-binding protein
    • Collagen prolyl 4 hydroxylase beta
    • Disulphide Isomerase
    • DSI
    • EC 5.3.4.1
    • Endoplasmic reticulum resident protein 59
    • ER protein 59
    • ERBA2L
    • ERp59
    • GIT
    • Gltathione insulin transhydrogenase
    • Glutathione insulin transhydrogenase
    • P4HB
    • P4Hbeta
    • p55
    • PDI
    • PDIA1
    • PDIA1_HUMAN
    • PDIR
    • PHDB
    • PO4DB
    • PO4HB
    • Procollagen proline 2 oxoglutarate 4 dioxygenase (proline 4 hydroxylase) beta polypeptide (protein disulfide isomerase associated 1)
    • Procollagen proline 2 oxoglutarate 4 dioxygenase beta subunit
    • PROHB
    • Prolyl 4 hydroxylase beta polypeptide
    • Prolyl 4 hydroxylase beta subunit
    • Prolyl 4 hydroxylase subunit beta
    • Prolyl 4-hydroxylase subunit beta
    • Protein disulfide isomerase associated 1
    • Protein disulfide isomerase, family A, member 1
    • Protein disulfide isomerase/oxidoreductase
    • Protein disulfide-isomerase
    • Protocollagen hydroxylase
    • Thbp
    • Thyroid hormone binding protein p55
    • Thyroid hormone binding protein p55 cellular
    • V erb a avian erythroblastic leukemia viral oncogene homolog 2 like
    see all
  • 功能
    This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP.
  • 序列相似性
    Belongs to the protein disulfide isomerase family.
    Contains 2 thioredoxin domains.
  • 细胞定位
    Endoplasmic reticulum lumen. Melanosome. Cell membrane. Highly abundant. In some cell types, seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources (Probable). Localizes near CD4-enriched regions on lymphoid cell surfaces. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

图片

  • SDS-PAGE analysis
    Lane 1: MW marker
    Lane 2: ab115719 at 0.5 µg
    Lane 3: ab115719 at 1 µg
    Lane 4: ab115719 at 2 µg
    Lane 5: ab115719 at 5 µg
  • Monoclonal mouse P4HB antibody + Recombinant Human P4HB protein (ab115719) at 0.05 µg

文献

ab115719 has not yet been referenced specifically in any publications.

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