Recombinant人p35 protein (ab119724)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • AccessionQ15078
    • 种属Human
    • 分子量60 kDa including tags
    • 氨基酸1 to 307

技术指标

Our Abpromise guarantee covers the use of ab119724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot

    SDS-PAGE

  • 纯度> 70 % SDS-PAGE.
    The purity was determined to be 70% by densitometry
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 25% Glycerol, 0.29% Sodium chloride, 0.79% Tris HCl, 0.003% EDTA, 0.003% DTT, 0.31% Glutathione, 0.002% PMSF

常规信息

  • 别名
    • CD5R1_HUMAN
    • CDK5 activator 1
    • CDK5P35
    • CDK5R
    • CDK5R1
    • Cyclin dependent kinase 5 regulatory subunit 1
    • Cyclin-dependent kinase 5 activator 1
    • Cyclin-dependent kinase 5 regulatory subunit 1
    • NCK 5A
    • Neuronal CDK5 activator
    • p23
    • p25
    • p35
    • Regulatory partner for CDK5 kinase
    • Tau protein kinase II 23 kDa subunit
    • TPKII regulatory subunit
    see all
  • 功能p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Activator of TPKII.
  • 组织特异性Brain and neuron specific.
  • 序列相似性Belongs to the cyclin-dependent kinase 5 activator family.
  • 翻译后修饰The p35 form is proteolytically cleaved by calpain, giving rise to the p25 form. P35 has a 5 to 10 fold shorter half-life compared to p25. The conversion results in deregulation of the CDK5 kinase: p25/CDK5 kinase displays an increased and altered tau phosphorylation in comparison to the p35/CDK5 kinase in vivo.
    Probably myristoylated. The Gly-2-Ala mutant is absent of the cell periphery, suggesting that a proper myristoylation signal is essential for the proper distribution of p35.
  • 细胞定位Cell membrane. In the primary cortical neurons, p35 is present in the peripheries and nerve terminals and Nucleus. Cytoplasm > perinuclear region. The conversion of p35 to p25 relocalizes the protein from the cell periphery to the cytoplasm, in nuclear and perinuclear regions. In the primary cortical neurons, p25 is primarily concentrated in the cell soma and is largely absent from neurites.
  • Information by UniProt

Recombinant Human p35 protein 图像

  • SDS Page analysis of ab119724.

Recombinant Human p35 protein (ab119724)参考文献

ab119724 has not yet been referenced specifically in any publications.

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