Recombinant人Occludin protein (ab114189)

概述

描述

  • 性质
    Recombinant
  • 来源
    Wheat germ
  • 氨基酸序列
    • Accession
    • 种属
      Human
    • 序列
      MSSRPLESPPPYRPDEFKPNHYAPSNDIYGGEMHVRPMLSQPAYSFYPED EILHFYKWTSPPGVIRILSMLIIVMCIAIFACVASTLAWDRGYGTSLLGG SVGYPYGGSGFGSYGSGYGYGYGYGYGYGGYTDPRAAKGFMLAMAAFCFI AALVIFVTSVIRSEMSRTRRYYLSVIIVSAILGIMVFIATIVYIMGVNPT AQSSGSLYGSQIYALCNQFYTPAATGLYVDQYSYHYCVVDPQEAIAIVLG FMIIVAFALIIFFAVKTRRKMDRYDKSNILWDKEHIYDEQPPNVEEWVKN VSAGTQDVPSPPSDYVERVDSPMAYSSNGKVNDKRFYPESSYKSTPVPEV VQELPLTSPVDDFRQPRYSSGGNFETPSKRAPAKGRAGRSKRTEQDHYET DYTTGGESCDELEEDWIREYPPITSDQQRQLYKRNFDTGLQEYKSLQSEL DEINKELSRLDKELDDYREESEEYMAAADEYNRLKQVKGSADYKSKKNHC KQLKSKLSHIKKMVGDYDRQKT
    • 分子量
      84 kDa including tags
    • 氨基酸
      1 to 522

相关产品

技术指标

Our Abpromise guarantee covers the use of ab114189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    Western blot

    ELISA

  • 形式
    Liquid
  • 补充说明
    Protein concentration is above or equal to 0.05 mg/ml.
    Best used within three months from the date of receipt.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.79% Tris HCl, 0.3% Glutathione

常规信息

  • 别名
    • BLCPMG
    • FLJ08163
    • FLJ18079
    • FLJ77961
    • FLJ94056
    • MGC34277
    • Occludin
    • Ocln
    • OCLN_HUMAN
    • Phosphatase 1 regulatory subunit 115
    • PPP1R115
    • PTORCH1
    • Tight junction protein occludin
    see all
  • 功能
    May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.
  • 组织特异性
    Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.
  • 疾病相关
    Defects in OCLN are the cause of band-like calcification with simplified gyration and polymicrogyria (BLCPMG) [MIM:251290]; also known as pseudo-TORCH syndrome. BLCPMG is a neurologic disorder with characteristic clinical and neuroradiologic features that mimic intrauterine TORCH infection in the absence of evidence of infection. Affected individuals have congenital microcephaly, intracranial calcifications, and severe developmental delay.
  • 序列相似性
    Belongs to the ELL/occludin family.
    Contains 1 MARVEL domain.
  • 结构域
    The C-terminal is cytoplasmic and is important for interaction with ZO-1. Sufficient for the tight junction localization. Involved in the regulation of the permeability barrier function of the tight junction (By similarity). The first extracellular loop participates in an adhesive interaction.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR. Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1.
  • 细胞定位
    Membrane. Cell junction > tight junction.
  • Information by UniProt

图片

  • ab114189 analysed on a 12.5% SDS-PAGE gel stained with Coomassie Blue.

文献

ab114189 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for your reply. I have processed your request to receive a new vial of ab114189 Occludin protein. At the moment the protein is out of stock and we will shop it to you as soon as it we can. Unfortunately, we do not have a estimated delivery da...

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Thank you for your patience while the lab was redoing the QC testing on ab114189. I just got the result from the QC department, please see the attachment, which has a copy of the western blot.As you could see, we used the anti-OCLN antibody and anti-GS...

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Thank you for contacting Abcam. I am sorry that you were having problems with ab114189, Occludin protein. I have asked the lab to redo the quality control on this product and see if there is a problem with the current whole batch of the protein, or if ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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