Recombinant人核Factor Erythroid Derived 2 protein (ab117042)

概述

描述

  • 性质Recombinant
  • 来源Wheat germ
  • 氨基酸序列
    • AccessionQ16621
    • 种属Human
    • 序列MSPCPPQQSRNRVIQLSTSELGEMELTWQEIMSITELQGLNAPSEPSFEP QAPAPYLGPPPPTTYCPCSIHPDSGFPLPPPPYELPASTSHVPDPPYSYG NMAIPVSKPLSLSGLLSEPLQDPLALLDIGLPAGPPKPQEDPESDSGLSL NYSDAESLELEGTEAGRRRSEYVEMYPVEYPYSLMPNSLAHSNYTLPAAE TPLALEPSSGPVRAKPTARGEAGSRDERRALAMKIPFPTDKIVNLPVDDF NELLARYPLTESQLALVRDIRRRGKNKVAAQNCRKRKLETIVQLERELER LTNERERLLRARGEADRTLEVMRQQLTELYRDIFQHLRDESGNSYSPEEY ALQQAADGTIFLVPRGTKMEATD
    • 分子量67 kDa including tags
    • 氨基酸1 to 373

技术指标

Our Abpromise guarantee covers the use of ab117042 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    ELISA

    SDS-PAGE

    Western blot

  • 形式Liquid
  • 补充说明Protein concentration is above or equal to 0.05 mg/ml.
    Best use within three months from the date of receipt of this protein.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

常规信息

  • 别名
    • erythroid-derived 2 45 kDa subunit
    • Leucine zipper protein NF-E2
    • Leucine zipper protein NFE2
    • NF E2
    • nfe2
    • NFE2_HUMAN
    • Nuclear factor
    • Nuclear factor (erythroid derived 2), 45kDa
    • nuclear factor (erythroid-derived 2), 45kD
    • nuclear factor, erythroid 2
    • Nuclear factor, erythroid derived 2 45 kDa subunit
    • p45
    • p45 NF-E2
    • p45 NFE2
    • Transcription factor NF-E2 45 kDa subunit
    • Transcription factor NFE2 45 kDa subunit
    see all
  • 功能Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.
  • 组织特异性Expressed in hematopoietic cells and also in colon and testis.
  • 序列相似性Belongs to the bZIP family. CNC subfamily.
    Contains 1 bZIP domain.
  • 结构域The PXY motifs are required for binding WW domains. PXY1 is required to promote transactivation of beta-globin and for hyperacetylation of histone H3, but not for binding to the HS2 promoter site.
  • 翻译后修饰Phosphorylated on serine residues (By similarity). In undifferentiated erythrocytes, phosphorylated by MAPK8 which then leads to ubiquitination and protein degradation.
    Sumoylated. Sumoylation is required for translocation to nuclear bodies PODs, anchoring to the gene loci, and transactivation of the beta-globin gene.
    Ubiquitinated mainly by 'Lys63'-linked ubiquitin. Polyubiquitination with 'Lys63'-linked ubiquitin by ITCH retains NFE2 in the cytoplasm preventing its transactivation activity. In undifferentiated erythrocyte, ubiquitinated after MAPK8-mediatd phosphorylation leading to protein degradation.
  • 细胞定位Nucleus > PML body. Cytoplasm. The sumoylated form locates to the nuclear bodies PML oncogenic domains (PODs). Translocated to the cytoplasm through interaction with ITCH.
  • Information by UniProt

Recombinant Human Nuclear Factor Erythroid Derived 2 protein 图像

  • 12.5% SDS-PAGE stained with Coomassie Blue showing ab117042 at approximately 66.77 kDa.

Recombinant Human Nuclear Factor Erythroid Derived 2 protein (ab117042)参考文献

ab117042 has not yet been referenced specifically in any publications.

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