重组人NUAK2蛋白(ab105209)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, WB, Functional Studies
描述
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产品名称
重组人NUAK2蛋白
参阅全部 NUAK2 蛋白酶 -
生物活性
The Specific activity of ab105209 was determined to be 80 nmol/min/mg.
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纯度
> 75 % SDS-PAGE.
Purity was determined to be >75% by densitometry. Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
110 kDa including tags -
氨基酸
1 to 628
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相关产品
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Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab105209 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Western blot
Functional Studies
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形式
Liquid -
补充说明
ab204854 (Cdc25C peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- DKFZp434J037
- DKFZp686F01113
- FLJ90349
see all -
功能
Stress-activated kinase involved in tolerance to glucose starvation. Induces cell-cell detachment by increasing F-actin conversion to G-actin. Expression is induced by CD95 or TNF-alpha, via NF-kappa-B. Protects cells from CD95-mediated apoptosis and is required for the increased motility and invasiveness of CD95-activated tumor cells. Able to phosphorylate 'Ser-464' of LATS1. -
序列相似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Phosphorylated at Thr-208 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Autophosphorylated in vitro. - Information by UniProt
图片
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The specific activity of NUAK2 (ab105209) was determined to be 11.2 nmol/min/mg as per activity assay protocol and was equivalent to 81 nmol/min/mg as per radiometric assay
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SDS PAGE analysis of ab105209
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SDS PAGE analysis of ab105209
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The Specific activity of ab105209 was determined to be 80 nmol/min/mg.
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SDS-PAGE showing ab105209 at approximately 110kDa.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab105209 尚未被引用在任何文献中。