Recombinant人NSE protein (ab78797)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 序列MSIEKIWARE ILDSRGNPTV EVDLYTAKGL FRAAVPSGAS TGIYEALELR DGDKQRYLGK GVLKAVDHIN STIAPALISS GLSVVEQEKL DNLMLELDGT ENKSKFGANA ILGVSLAVCK AGAAERELPL YRHIAQLAGN SDLILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAESFRD AMRLGAEVYH TLKGVIKDKY GKDATNVGDE GGFAPNILEN SEALELVKEA IDKAGYTEKI VIGMDVAASE FYRDGKYDLD FKSPTDPSRY ITGDQLGALY QDFVRDYPVV SIEDPFDQDD WAAWSKFTAN VGIQIVGDDL TVTNPKRIER AVEEKACNCL LLKVNQIGSV TEAIQACKLA QENGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL MRIEEELGDE ARFAGHNFRN PSVL

技术指标

Our Abpromise guarantee covers the use of ab78797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot

    SDS-PAGE

  • 内毒素水平< 1.000 Eu/µg
  • 纯度> 95 % SDS-PAGE.
    Purity is > 98% by SDS PAGE. ab78797 is purified using conventional chromatography techniques.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 0.1M Potassium chloride, 20mM Tris, 5mM Magnesium sulphate, pH 7.5

常规信息

  • 别名
    • 2 phospho D glycerate hydrolyase
    • 2-phospho-D-glycerate hydro-lyase
    • Eno 2
    • ENO2
    • ENOG
    • ENOG_HUMAN
    • Enolase 2
    • Enolase 2 (gamma, neuronal)
    • Enolase 2 gamma neuronal
    • Enolase2
    • Epididymis secretory protein Li 279
    • Gamma enolase
    • Gamma-enolase
    • HEL S 279
    • Neural enolase
    • Neuron specific enolase
    • Neuron specific gamma enolase
    • Neuron-specific enolase
    • Neurone specific enolase
    • NSE
    see all
  • 功能Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
  • 组织特异性The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
  • 通路Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
  • 序列相似性Belongs to the enolase family.
  • 发展阶段During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
  • 细胞定位Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
  • Information by UniProt

Recombinant Human NSE protein 图像

  • Anti-NSE antibody (ab90473) at 1 µg/ml + Recombinant Human NSE protein (ab78797) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 4 minutes
  • 15% SDS-PAGE showing ab78797 at approximately 47kDa (3µg).

Recombinant Human NSE protein (ab78797)参考文献

ab78797 has not yet been referenced specifically in any publications.

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We recommend storing the protein in 10ul aliquots at -20C for longer term.

Although we haven't used these proteins in functional assays however you can try using these proteins either by diluting in cul...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"