The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Unit Definition: One unit will catalyse the reduction of 1.0 micromole of Cytochrome C per minute in the presence of Menadione and 1-Carbamoylmethyl-3 carbamoyl-1,4-dihydropyrimidine at pH 7.4 at 37°C.
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
Belongs to the NAD(P)H dehydrogenase (quinone) family.