Recombinant人NOTCH3 protein (ab114234)

概述

描述

  • 性质Recombinant
  • 来源Wheat germ
  • 氨基酸序列
    • AccessionQ9UM47
    • 种属Human
    • 序列SPCANGGRCTQLPSREAACLCPPGWVGERCQLEDPCHSGPCAGRGVCQSS VVAGTARFSCRCPRGFRGPDCSLPD PCLSSPCAHGARCSVGPDGRFLC SCPPGYQGRSCR
    • 分子量38 kDa including tags
    • 氨基酸47 to 156

技术指标

Our Abpromise guarantee covers the use of ab114234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    ELISA

    SDS-PAGE

    Western blot

  • 形式Liquid
  • 补充说明Protein concentration is above or equal to 0.05 mg/ml
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

常规信息

  • 别名
    • CADASIL
    • CASIL
    • NOTC3_HUMAN
    • Notch 3
    • Notch 3 intracellular domain
    • Notch homolog 3
    • Notch3
    see all
  • 功能Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.
  • 组织特异性Ubiquitously expressed in fetal and adult tissues.
  • 疾病相关Defects in NOTCH3 are the cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) [MIM:125310]. CADASIL causes a type of stroke and dementia of which key features include recurrent subcortical ischemic events and vascular dementia. The disorder affects relatively young adults of both sexes. Mutations affect highly conserved cysteine residues within epidermal growth factor (EGF)-like repeat domains in the extracellular part of the receptor.
  • 序列相似性Belongs to the NOTCH family.
    Contains 5 ANK repeats.
    Contains 34 EGF-like domains.
    Contains 3 LNR (Lin/Notch) repeats.
  • 翻译后修饰Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
    Phosphorylated.
  • 细胞定位Cell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus.
  • Information by UniProt

Recombinant Human NOTCH3 protein 图像

  • 12.5% SDS-PAGE showing ab114234 at approximately 37.73kDa stained with Coomassie Blue.

Recombinant Human NOTCH3 protein (ab114234)参考文献

ab114234 has not yet been referenced specifically in any publications.

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