Recombinant人NIPP1 protein (ab114902)

概述

描述

  • 性质Recombinant
  • 来源Wheat germ
  • 氨基酸序列
    • AccessionQ12972
    • 种属Human
    • 序列RRMQNFAFSGGLYGGLPPTHSEAGSQPHGIHGTALIGGLPMPYPNLAPDV DLTPVVPSAVNMNPAPNPAVYNPEAVNEPKKKKYAKEAWPGKKPTPSLLI
    • 分子量37 kDa including tags
    • 氨基酸28 to 127

技术指标

Our Abpromise guarantee covers the use of ab114902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot

    SDS-PAGE

    ELISA

  • 形式Liquid
  • 补充说明Protein concentration is above or equal to 0.05 mg/ml. Best use within three months from the date of receipt of this protein.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

常规信息

  • 别名
    • Activator of RNA decay
    • ARD 1
    • ARD-1
    • ARD1
    • Homolog of E.coli RNase E
    • NIPP 1
    • NIPP-1
    • NIPP1
    • Nuclear inhibitor of protein phosphatase 1
    • nuclear inhibitor of protein phosphatase-1 alpha
    • Nuclear subunit of PP1
    • PP1R8_HUMAN
    • PPP1R8
    • PRO2047
    • Protein phosphatase 1 regulatory inhibitor subunit 8
    • Protein phosphatase 1 regulatory subunit 8
    • RNase E
    • RNase E, E. coli, homolog of
    see all
  • 功能Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation.
    Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3' to purines and pyrimidines in A+U-rich regions. It generates 5'-phosphate termini at the site of cleavage. This isoform does not inhibit PP-1. May be implicated in mRNA splicing.
  • 组织特异性Ubiquitously expressed, with highest levels in heart and skeletal muscle, followed by brain, placenta, lung, liver and pancreas. Less abundant in kidney. The concentration and ratio between isoforms is cell-type dependent. Isoform Alpha (>90%) and isoform Beta were found in brain, heart and kidney. Isoform Gamma is mainly found in B-cells and T-lymphocytes, and has been found in 293 embryonic kidney cells.
  • 序列相似性Contains 1 FHA domain.
  • 结构域Has a basic N- and C-terminal and an acidic central domain.
  • 翻译后修饰May be inactivated by phosphorylation on Ser-199 or Ser-204 (By similarity). Phosphorylated by Lyn in vitro on Tyr-264, and also on Tyr-335 in the presence of RNA.
  • 细胞定位Nucleus. Nucleus speckle. Primarily, but not exclusively, nuclear and Cytoplasm. Found mainly in the cytoplasm.
  • Information by UniProt

Recombinant Human NIPP1 protein 图像

  • 12.5% SDS-PAGE showing ab114902 at approximately 36.63kDa.
    Stained with Coomassie Blue.

Recombinant Human NIPP1 protein (ab114902)参考文献

ab114902 has not yet been referenced specifically in any publications.

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