Recombinant人NFkB p105 / p50 protein (ab112327)

概述

描述

  • 性质Recombinant
  • 来源Wheat germ
  • 氨基酸序列
    • AccessionP19838-2
    • 种属Human
    • 序列MDNYEVSGGTVRELVEALRQMGYTEAIEVIQAASSPVKTTSQAHSLPLSP ASTRQQIDELRDSDSVCDSGVETSFRKLSFTESLTSGASLLTLNKMPHDY GQEGPLEGKI
    • 分子量38 kDa including tags
    • 氨基酸860 to 969
    • 标签GST tag N-Terminus

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技术指标

Our Abpromise guarantee covers the use of ab112327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot

    ELISA

    SDS-PAGE

  • 形式Liquid
  • 补充说明

    Best use within three months from the date of receipt of this protein.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.79% Tris HCl, 0.31% Glutathione

常规信息

  • 别名
    • DKFZp686C01211
    • DNA binding factor KBF1
    • DNA binding factor KBF1 EBP1
    • DNA-binding factor KBF1
    • EBP 1
    • EBP-1
    • EBP1
    • KBF1
    • MGC54151
    • NF kappa B
    • NF kappaB
    • NF kappabeta
    • NF kB1
    • NFkappaB
    • NFKB 1
    • NFKB p105
    • NFKB p50
    • Nfkb1
    • NFKB1_HUMAN
    • Nuclear factor kappa B DNA binding subunit
    • Nuclear factor kappa-B, subunit 1
    • Nuclear factor NF kappa B p105 subunit
    • Nuclear factor NF kappa B p50 subunit
    • Nuclear factor NF-kappa-B p50 subunit
    • Nuclear factor of kappa light chain gene enhancer in B cells 1
    • Nuclear factor of kappa light polypeptide gene enhancer in B cells 1
    • Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
    • p105
    • p50
    • p84/NF-kappa-B1 p98
    • Transcription factor NFKB1
    see all
  • 功能NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105.
  • 序列相似性Contains 7 ANK repeats.
    Contains 1 death domain.
    Contains 1 RHD (Rel-like) domain.
  • 结构域The C-terminus of p105 might be involved in cytoplasmic retention, inhibition of DNA-binding, and transcription activation.
    Glycine-rich region (GRR) appears to be a critical element in the generation of p50.
  • 翻译后修饰While translation occurs, the particular unfolded structure after the GRR repeat promotes the generation of p50 making it an acceptable substrate for the proteasome. This process is known as cotranslational processing. The processed form is active and the unprocessed form acts as an inhibitor (I kappa B-like), being able to form cytosolic complexes with NF-kappa B, trapping it in the cytoplasm. Complete folding of the region downstream of the GRR repeat precludes processing.
    Phosphorylation at 'Ser-903' and 'Ser-907' primes p105 for proteolytic processing in response to TNF-alpha stimulation. Phosphorylation at 'Ser-927' and 'Ser-932' are required for BTRC/BTRCP-mediated proteolysis.
    Polyubiquitination seems to allow p105 processing.
    S-nitrosylation of Cys-61 affects DNA binding.
  • 细胞定位Nucleus. Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor.
  • Information by UniProt

Recombinant Human NFkB p105 / p50 protein 图像

  • ab112327 analysed on a 12.5% SDS-PAGE gel stained with Coomassie Blue.

Recombinant Human NFkB p105 / p50 protein (ab112327)参考文献

ab112327 has not yet been referenced specifically in any publications.

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