Recombinant人NFAT2 protein (ab64307)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • 种属Human

技术指标

Our Abpromise guarantee covers the use of ab64307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot

    SDS-PAGE

  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Sodium chloride, 50mM Tris HCl, 0.25mM DTT, 0.1mM PMSF, pH 7.5

常规信息

  • 别名
    • cytoplasmic 1
    • MGC138448
    • NF ATc
    • NF ATc1
    • NF-ATc
    • NF-ATc1
    • NF-ATc1.2
    • NFAC1_HUMAN
    • NFAT 2
    • NFAT transcription complex cytosolic component
    • NFATc
    • NFATC 1
    • NFATc1
    • Nuclear factor of activated T cells cytoplasmic 1
    • Nuclear factor of activated T cells cytoplasmic calcineurin dependent 1
    • Nuclear factor of activated T cells cytosolic component 1
    • Nuclear factor of activated T-cells
    • nuclear factor of activated T-cells 'c'
    see all
  • 功能Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells.
  • 组织特异性Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T-cells after second antigen exposure.
  • 序列相似性Contains 1 RHD (Rel-like) domain.
  • 结构域Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.
    The N-terminal transactivation domain (TAD-A) binds to and is activated by Cbp/p300. The dephosphorylated form contains two unmasked nuclear localization signals (NLS), which allow translocation of the protein to the nucleus.
    Isoforms C have a C-terminal part with an additional trans-activation domain, TAD-B, which acts as a transcriptional activator. Isoforms B have a shorter C-terminal part without complete TAD-B which acts as a transcriptional repressor.
  • 翻译后修饰Phosphorylated by NFATC-kinase; dephosphorylated by calcineurin.
  • 细胞定位Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
  • Information by UniProt

Recombinant Human NFAT2 protein 图像

  • ab64307 on SDS-PAGE, MW 125kDa.

Recombinant Human NFAT2 protein (ab64307)参考文献

ab64307 has not yet been referenced specifically in any publications.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"