Recombinant人Neuroglobin protein (ab63278)

概述

  • 产品名称Recombinant人Neuroglobin protein
  • 蛋白长度Full length protein

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 序列M-ERPEPELIR QSWRAVSRSP LEHGTVLFAR LFALEPDLLP LFQYNCRQFS SPEDCLSSPE FLDHIRKVML VIDAAVTNVE DLSSLEEYLA SLGRKHRAVG VKLSSFSTVG ESLLYMLEKC LGPAFTPATR AAWSQLYGAV VQAMSRGWDG E

相关产品

技术指标

Our Abpromise guarantee covers the use of ab63278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot

    ELISA

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.
    Please filter the product by an appropriate sterile filter before using it in the cell culture.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 0.1M Sodium chloride, 0.05M PBS, pH 7.2

常规信息

  • 别名
    • Neuroglobin
    • NGB
    • NGB_HUMAN
  • 功能Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes. The deoxygenated form exhibits nitrite reductase activity inhibiting cellular respiration via NO-binding to cytochrome c oxidase. Involved in neuroprotection during oxidative stress. May exert its anti-apoptotic activity by acting to reset the trigger level of mitochondrial cytochrome c release necessary to commit the cells to apoptosis.
  • 组织特异性Predominantly expressed in brain, the strongest expression is seen in the frontal lobe, the subthalamic nucleus and the thalamus.
  • 序列相似性Belongs to the globin family.
  • 翻译后修饰A redox disulfide bond regulates the heme pocket coordination and the rate of nitrite reduction to NO.
    Phosphorylated in vitro by ERK1, ERK2 and PKA, and in vivo during hypoxia. Phosphorylation increases nitrite reductase activity.
  • 细胞定位Perikaryon. Cytoplasm. Mitochondrion.
  • Information by UniProt

Recombinant Human Neuroglobin protein 图像

  • LANE 1. M.W. marker - 14, 21, 31, 45, 66, 97 kDa. LANE 2. reduced and heated sample, 5µg/lane. LANE 3. non-reduced and non-heated sample, 5µg/lane.
  • Anti-Neuroglobin antibody [13C8] (ab37258) at 1 µg/ml + Recombinant Human Neuroglobin protein (ab63278) at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 4 minutes

Recombinant Human Neuroglobin protein (ab63278)参考文献

ab63278 has not yet been referenced specifically in any publications.

Product Wall

Perdona por la espera. Esta proteína no contiene el grupo hemo. Si tienes más preguntas, por favor, no dudes en contactarnos.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"