Recombinant人NEK9 protein (ab125614)

概述

描述

  • 性质
    Recombinant
  • 来源
    Baculovirus infected Sf9 cells
  • 氨基酸序列
    • Accession
    • 种属
      Human
    • 分子量
      115 kDa including tags
    • 氨基酸
      1 to 979
    • 标签
      GST tag N-Terminus
    • 额外的序列信息
      1-979; deleted fragment of 346-731

相关产品

技术指标

Our Abpromise guarantee covers the use of ab125614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性
    The specific activity of ab125614 was determined to be 120 nmol/mg/min.
  • 应用

    Western blot

    Functional Studies

    SDS-PAGE

  • 纯度
    > 75 % SDS-PAGE.
    Purity determined to be >75% by densitometry.
  • 形式
    Liquid
  • 补充说明

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.004% EGTA, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • KIAA1995
    • APUG
    • DKFZp434D0935
    • LCCS10
    • MGC138306
    • MGC16714
    • NC
    • NEK 9
    • Nek8
    • nek9
    • NEK9_HUMAN
    • NERCC
    • Nercc 1 kinase
    • NERCC1
    • Nercc1 kinase
    • Never in mitosis A related kinase 9
    • Never in mitosis A-related kinase 9
    • NIMA (never in mitosis gene a) related kinase 9
    • NIMA related kinase 8
    • NIMA related kinase 9
    • NIMA related kinase Nek8
    • NimA related protein kinase 9
    • NIMA-related kinase 8
    • NimA-related protein kinase 9
    • Serine/threonine protein kinase Nek9
    • Serine/threonine-protein kinase Nek9
    see all
  • 功能
    Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression.
  • 组织特异性
    Most abundant in heart, liver, kidney and testis. Also expressed in smooth muscle cells and fibroblasts.
  • 序列相似性
    Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
    Contains 1 protein kinase domain.
    Contains 6 RCC1 repeats.
  • 发展阶段
    Expression varied mildly across the cell cycle, with highest expression observed in G1 and stationary-phase cells.
  • 结构域
    Dimerizes through its coiled-coil domain.
  • 翻译后修饰
    Autophosphorylated on serine and threonine residues. When complexed with FACT, exhibits markedly elevated phosphorylation on Thr-210. During mitosis, not phosphorylated on Thr-210. Phosphorylated by CDK1 in vitro.
  • 细胞定位
    Cytoplasm. Nucleus.
  • Information by UniProt

图片

  • Sample Kinase Assay showing the specific activity of ab125614 as 120 nmol/min/mg.
  • SDS-PAGE analysis of ab125614.

文献

ab125614 has not yet been referenced specifically in any publications.

客户评价及客户问答

There are currently no Customer reviews or Questions for ab125614.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册