Recombinant人NEK9 protein (ab125614)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • AccessionNM_033116
    • 种属Human
    • 分子量115 kDa including tags
    • 氨基酸1 to 979
    • 标签GST tag N-Terminus
    • 额外的序列信息1-979; deleted fragment of 346-731

技术指标

Our Abpromise guarantee covers the use of ab125614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性The specific activity of ab125614 was determined to be 120 nmol/mg/min.
  • 应用

    Western blot

    Functional Studies

    SDS-PAGE

  • 纯度> 75 % SDS-PAGE.
    Purity determined to be >75% by densitometry.
  • 形式Liquid
  • 补充说明

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.004% EGTA, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • KIAA1995
    • APUG
    • DKFZp434D0935
    • LCCS10
    • MGC138306
    • MGC16714
    • NC
    • NEK 9
    • Nek8
    • nek9
    • NEK9_HUMAN
    • NERCC
    • Nercc 1 kinase
    • NERCC1
    • Nercc1 kinase
    • Never in mitosis A related kinase 9
    • Never in mitosis A-related kinase 9
    • NIMA (never in mitosis gene a) related kinase 9
    • NIMA related kinase 8
    • NIMA related kinase 9
    • NIMA related kinase Nek8
    • NimA related protein kinase 9
    • NIMA-related kinase 8
    • NimA-related protein kinase 9
    • Serine/threonine protein kinase Nek9
    • Serine/threonine-protein kinase Nek9
    see all
  • 功能Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression.
  • 组织特异性Most abundant in heart, liver, kidney and testis. Also expressed in smooth muscle cells and fibroblasts.
  • 序列相似性Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
    Contains 1 protein kinase domain.
    Contains 6 RCC1 repeats.
  • 发展阶段Expression varied mildly across the cell cycle, with highest expression observed in G1 and stationary-phase cells.
  • 结构域Dimerizes through its coiled-coil domain.
  • 翻译后修饰Autophosphorylated on serine and threonine residues. When complexed with FACT, exhibits markedly elevated phosphorylation on Thr-210. During mitosis, not phosphorylated on Thr-210. Phosphorylated by CDK1 in vitro.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt

Recombinant human NEK9 protein 图像

  • Sample Kinase Assay showing the specific activity of ab125614 as 120 nmol/min/mg.
  • SDS-PAGE analysis of ab125614.

Recombinant human NEK9 protein (ab125614)参考文献

ab125614 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab125614.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"