Recombinant人NEK6 protein (ab42600)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • 种属Human
    • 序列MAGQPGHMPH GGSSNNLCHT LGPVHPPDPQ RHPNTLSFRC SLADFQIEKK IGRGQFSEVY KATCLLDRKT VALKKVQIFE MMDAKARQDC VKEIGLLKQL NHPNIIKYLD SFIEDNELNI VLELADAGDL SQMIKYFKKQ KRLIPERTVW KYFVQLCSAV EHMHSRRVMH RDIKPANVFI TATGVVKLGD LGLGRFFSSE TTAAHSLVGT PYYMSPERIH ENGYNFKSDI WSLGCLLYEM AALQSPFYGD KMNLFSLCQK IEQCDYPPLP GEHYSEKLRE LVSMCICPDP HQRPDIGYVH QVAKQMHIWM SST

技术指标

Our Abpromise guarantee covers the use of ab42600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Activity: 70 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1 nmol NEK6 substrate (FLAKSFGSPNRAYKK) per minute at pH 7.4 and 30C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Naorthovanadate, 0.1 mM ATP, 4 uM NEK6 substrate, and 0.4 mg/ml NEK6.
  • 应用

    Inhibition Assay

  • 纯度> 90 % SDS-PAGE.
    >90% by SDS-PAGE
  • 形式Liquid
  • 补充说明Expressed in a Baculovirus infected Sf9 cell expression system.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Highly similar to cell cycle protein kinase CDC5/MSD2
    • NEK6
    • NEK6_HUMAN
    • Never in mitosis A-related kinase 6
    • NIMA (Never In Mitosis Gene A) Related Kinase 6
    • NimA related protein kinase 6
    • NimA-related protein kinase 6
    • Protein kinase SID6 1512
    • Protein kinase SID6-1512
    • Putative serine threonine protein kinase
    • Serine/threonine protein kinase Nek6
    • Serine/threonine-protein kinase Nek6
    • SID6 1512
    • SID61512
    see all
  • 功能Activated during M phase. Required for chromosome segregation at metaphase-anaphase transition and therefore for mitotic progression. Inhibition of activity results in apoptosis. Phosphorylates KIF11 to promote mitotic spindle formation.
  • 组织特异性Ubiquitous, with highest expression in heart and skeletal muscle.
  • 序列相似性Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt

Recombinant human NEK6 protein (ab42600)参考文献

ab42600 has not yet been referenced specifically in any publications.

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