Recombinant人NEDD8 protein (ab87008)

概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • 种属
      Human
    • 序列
      MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMLI KVKTLTGKEI EIDIEPTDKV ERIKERVEEK EGIPPQQQRL IYSGKQMNDE KTAADYKILG GSVLHLVLAL RGG

技术指标

Our Abpromise guarantee covers the use of ab87008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

  • 纯度
    > 95 % SDS-PAGE.
    ab87008 is purified using conventional chromatography techniques, after refolding of the isolated inclusion body in renaturation buffer.
  • 形式
    Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 50mM Sodium chloride, 20mM Tris HCl, 0.5mM DTT, pH 8.0

常规信息

  • 别名
    • FLJ43224
    • MGC104393
    • MGC125896
    • MGC125897
    • NED8
    • NEDD 8
    • NEDD-8
    • Nedd8
    • NEDD8_HUMAN
    • Neddylin
    • Neural precursor cell expressed developmentally down regulated 8
    • Neural precursor cell expressed developmentally down regulated gene 8
    • Neural precursor cell expressed developmentally down-regulated protein 8
    • Rub1
    • Ubiquitin like protein Nedd 8
    • Ubiquitin like protein Nedd8
    • Ubiquitin-like protein Nedd8
    see all
  • 功能
    Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
  • 组织特异性
    Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.
  • 序列相似性
    Belongs to the ubiquitin family.
  • 翻译后修饰
    Cleavage of precursor form by UCHL3 or SENP8 is necessary for function.
  • 细胞定位
    Nucleus. Mainly nuclear.
  • Information by UniProt

图片

  • 15% SDS-PAGE analysis of 3µg ab87008

文献

ab87008 has not yet been referenced specifically in any publications.

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