Recombinant人NEDD8 protein (ab117668)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionQ15843
    • 种属Human
    • 序列MHHHHHHMKI EEGKLVIWIN GDKGYNGLAE VGKKFEKDTG IKVTVEHPDK LEEKFPQVAA TGDGPDIIFW AHDRFGGYAQ SGLLAEITPD KAFQDKLYPF TWDAVRYNGK LIAYPIAVEA LSLIYNKDLL PNPPKTWEEI PALDKELKAK GKSALMFNLQ EPYFTWPLIA ADGGYAFKYE NGKYDIKDVG VDNAGAKAGL TFLVDLIKNK HMNADTDYSI AEAAFNKGET AMTINGPWAW SNIDTSKVNY GVTVLPTFKG QPSKPFVGVL SAGINAASPN KELAKEFLEN YLLTDEGLEA VNKDKPLGAV ALKSYEEELA KDPRIAATME NAQKGEIMPN IPQMSAFWYA VRTAVINAAS GRQTVDEALK DAQTNSSSNN NNNNNNNNLG IEGRGSHMAA AEAANCIMEV SCGQAESSEK PNAEDMTSKD YYFDSYAHFG IHEEMLKDEV RTLTYRNSMF HNRHLFKDKV VLDVGSGTGILCMFAAKAGA RKVIGIECSS ISDYAVKIVK ANKLDHVVTI IKGKVEEVEL PVEKVDIIIS EWMGYCLFYE SMLNTVLHAR DKWLAPDGLI FPDRATLYVT AIEDRQYKDY KIHWWENVYG FDMSCIKDVA IKEPLVDVVD PKQLVTNACL IKEVDIYTVK VEDLTFTSPF CLQVKRNDYVHALVAYFNIE FTRCHKRTGF STSPESPYTH WKQTVFYMED YLTVKTGEEI FGTIGMRPNA KNNRDLDFTI DLDFKGQLCE LSCSTDYRMR
    • 分子量13 kDa including tags
    • 氨基酸1 to 76
    • 标签His tag N-Terminus
    • 额外的序列信息NEDD8 Human Recombinant fused with 37 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 113 amino acids (1-76 a.a.).

技术指标

Our Abpromise guarantee covers the use of ab117668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.
    ab117668 is purified by proprietary chromatographic techniques.
  • 形式Liquid
  • 补充说明Although stable at 4°C for 4 weeks, ab117668 should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Please see notes section.

    pH: 8.00
    Constituents: 0.24% Tris, 0.08% DTT, 10% Glycerol, 0.29% Sodium chloride

常规信息

  • 别名
    • FLJ43224
    • MGC104393
    • MGC125896
    • MGC125897
    • NED8
    • NEDD 8
    • NEDD-8
    • Nedd8
    • NEDD8_HUMAN
    • Neddylin
    • Neural precursor cell expressed developmentally down regulated 8
    • Neural precursor cell expressed developmentally down regulated gene 8
    • Neural precursor cell expressed developmentally down-regulated protein 8
    • Rub1
    • Ubiquitin like protein Nedd 8
    • Ubiquitin like protein Nedd8
    • Ubiquitin-like protein Nedd8
    see all
  • 功能Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
  • 组织特异性Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.
  • 序列相似性Belongs to the ubiquitin family.
  • 翻译后修饰Cleavage of precursor form by UCHL3 or SENP8 is necessary for function.
  • 细胞定位Nucleus. Mainly nuclear.
  • Information by UniProt

Recombinant Human NEDD8 protein (ab117668)参考文献

ab117668 has not yet been referenced specifically in any publications.

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