概述

描述

  • 性质
    Recombinant
  • 来源
    Wheat germ
  • 氨基酸序列
    • Accession
    • 种属
      Human
    • 序列
      HTKYKNGYSANHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAE LYGSNGPQSFTVEQWGTPEKLPRAHTCFNRLDLPPYESFEELWDKLQMAI ENTQGFDGVD
    • 分子量
      38 kDa including tags
    • 氨基酸
      791 to 900

技术指标

Our Abpromise guarantee covers the use of ab152562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    ELISA

    Western blot

    SDS-PAGE

  • 形式
    Liquid
  • 补充说明
    Protein concentration is above or equal to 0.05 µg/µl.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl

常规信息

  • 别名
    • Cell proliferation-inducing gene 53 protein
    • E3 ubiquitin protein ligase Nedd4
    • E3 ubiquitin-protein ligase NEDD4
    • HECT-type E3 ubiquitin transferase NEDD4
    • KIAA0093
    • NEDD 4
    • NEDD-4
    • Nedd4
    • NEDD4_HUMAN
    • Neural precursor cell expressed developmentally down regulated 4
    • Neural precursor cell expressed developmentally down-regulated protein 4
    • neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase
    • PIG53
    • receptor-potentiating factor 1
    • RPF1
    see all
  • 功能
    E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. According to PubMed:18562292 the direct link between NEDD4 and PTEN regulation through polyubiquitination described in PubMed:17218260 is questionable. Involved in ubiquitination of ERBB4 intracellular domain E4ICD. Involved in the budding of many viruses. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2.
  • 通路
    Protein modification; protein ubiquitination.
  • 序列相似性
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 4 WW domains.
  • 结构域
    The WW domains mediate interaction with LITAF, RNF11, WBP1, WBP2, TMEPAI, NDFIP1 and PRRG2.
  • 翻译后修饰
    Auto-ubiquitinated.
  • 细胞定位
    Cytoplasm. Cell membrane. Recruited to the plasma membrane by GRB10. Once complexed with GRB10 and IGF1R, follows IGF1R internalization, remaining associated with early endosomes. Uncouples from IGF1R-containing endosomes before the sorting of the receptor to the lysosomal compartment (By similarity). May be recruited to exosomes by NDFIP1.
  • Information by UniProt

图片

  • 12.5% SDS-PAGE analysis of ab152562 stained with Coomassie Blue.

文献

ab152562 has not yet been referenced specifically in any publications.

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