Recombinant人NCF1 protein (ab98054)

概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • Accession
    • 种属
      Human
    • 序列
      MGDTFIRHIALLGFEKRFVPSQHYVYMFLVKWQDLSEKVVYRRFTEIYEF HKTLKEMFPIEAGAINPENRIIPHLPAPKWFDGQRAAENRQGTLTEYCST LMSLPTKISRCPHLLDFFKVRPDDLKLPTDNQTKKPETYLMPKDGKSTAT DITGPIILQTYRAIANYEKTSGSEMALSTGDVVEVVEKSESGWWFCQMKA KRGWIPASFLEPLDSPDETEDPEPNYAGEPYVAIKAYTAVEGDEVSLLEG EAVEVIHKLLDGWWVIRKDDVTGYFPSMYLQKSGQDVSQAQRQIKRGAPP RRSSIRNAHSIHQRSRKRLSQDAYRRNSVRFLQQRRRQARPGPQSPGSPL EEERQTQRSKPQPAVPPRPSADLILNRCSESTKRKLASAVVEHHHHHH
    • 分子量
      46 kDa including tags
    • 氨基酸
      1 to 390
    • 标签
      His tag C-Terminus

技术指标

Our Abpromise guarantee covers the use of ab98054 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    Mass Spectrometry

    Western blot

  • 质谱法
    MALDI-TOF
  • 纯度
    > 90 % SDS-PAGE.
    ab98054 was purified using conventional chromatography techniques.
  • 形式
    Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 20% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, 1mM DTT, pH 8.0

常规信息

  • 别名
    • 47 kDa autosomal chronic granulomatous disease protein
    • 47 kDa neutrophil oxidase factor
    • NADPH oxidase organizer 2
    • NCF 47K
    • NCF-1
    • NCF-47K
    • Ncf1
    • NCF1_HUMAN
    • Neutrophil cytosol factor 1
    • Neutrophil cytosolic factor 1
    • neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1)
    • Neutrophil NADPH oxidase factor 1
    • Nox organizer 2
    • Nox organizing protein 2
    • Nox-organizing protein 2
    • NOXO2
    • p47 phox
    • p47-phox
    • SH3 and PX domain containing protein 1A
    • SH3 and PX domain-containing protein 1A
    • SH3PXD1A
    see all
  • 功能
    NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
  • 疾病相关
    Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
  • 序列相似性
    Contains 1 PX (phox homology) domain.
    Contains 2 SH3 domains.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt

图片

  • Anti-NCF1 antibody (ab795) at 1.5 µg/ml + Recombinant Human NCF1 protein (ab98054) at 0.1 µg

    Secondary
    Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 8 minutes
  • 15% SDS-PAGE analysis of 3µg ab98054

文献

ab98054 has not yet been referenced specifically in any publications.

客户评价及客户问答

Vielen Dank für Ihre Anfrage. Gegen die genannten NADPH-Oxidase-Komponenten haben wir folgende Antikörper bzw. Proteine in unserem Katalog: Click here (or use the following: http://www.abcam.com/index.html?datasheet=109366). Click here (or use the...

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