Recombinant人Nanog protein (ab50053)

概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • 种属
      Human
    • 序列
      SVDPACPQSL PCFEASDCKE SSPMPVICGP EENYPSLQMS SAEMPHTETV SPLPSSMDLL IQDSPDSSTS PKGKQPTSAE NSVAKKEDKV PVKKQKTRTV FSSTQLCVLN DRFQRQKYLS LQQMQELSNI LNLSYKQVKT WFQNQRMKSK RWQKNNWPKN SNGVTQKASA PTYPSLYSSY HQGCLVNPTG NLPMWSNQTW NNSTWSNQTQ NIQSWSNHSW NTQTWCTQSW NNQAWNSPFY NCGEESLQSC MQFQPNSPAS DLEAALEAAG EGLNVIQQTT RYFSTPQTMD LFLNYSMNMQ PEDV
    • 分子量
      35 kDa
    • 氨基酸
      1 to 304

技术指标

Our Abpromise guarantee covers the use of ab50053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

  • 内毒素水平
    < 0.100 Eu/µg
  • 纯度
    > 95 % SDS-PAGE.

  • 形式
    Lyophilised
  • 补充说明
    Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.

    Preservative: None
    Endotoxin level is less than 0.1 ng per µg (1EU/µg).

  • 复溶
    Reconstitute to 1mg/ml using 10mM Acetic acid.

常规信息

  • 别名
    • Embryonic stem cell specific homeobox protein (Nanog)
    • ENK
    • FLJ12581
    • hNanog
    • Homeobox protein NANOG
    • Homeobox transcription factor Nanog
    • homeobox transcription factor Nanog-delta 48
    • NANOG
    • Nanog homeobox
    • NANOG_HUMAN
    see all
  • 功能
    Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
  • 组织特异性
    Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
  • 序列相似性
    Belongs to the Nanog homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 发展阶段
    Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
  • 细胞定位
    Nucleus.
  • Information by UniProt

Recombinant Human Nanog protein 图像

Recombinant Human Nanog protein (ab50053)参考文献

This product has been referenced in:
  • Yang WC  et al. Cell-free production of transducible transcription factors for nuclear reprogramming. Biotechnol Bioeng 104:1047-58 (2009). ICC/IF, ELISA . Read more (PubMed: 19718703) »

See 1 Publication for this product

Product Wall

Thank you for your patience.

I have obtained the following additional information from the lab:

1) The purification details are proprietary, but 3 ion-exchange columns and no antibody or affinity columns were used.

2) Th...

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Thank you for contacting us.

The current information I have received from the supplying lab is as follows:

Unfortunately, the purification details are proprietary. There were no tags used in the expression of this protein.
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