Recombinant人Nanog protein (ab109960)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionQ9H9S0
    • 种属Human
    • 序列MGSSHHHHHHSSGLVPRGSHMSVDPACPQSLPCFEASDCKESSPMPVICG PEENYPSLQMSSAEMPHTETVSPLPSSMDLLIQDSPDSSTSPKGKQPTSA EKSVAKKEDKVPVKKQKTRTVFSSTQLCVLNDRFQRQKYLSLQQMQELSN ILNLSYKQVKTWFQNQRMKSKRWQ
    • 分子量20 kDa including tags
    • 氨基酸1 to 154
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab109960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    Mass Spectrometry

  • 质谱法
    MALDI-TOF
  • 纯度> 90 % SDS-PAGE.
    ab109960 was purified using conventional chromatography.
  • 形��Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 20% Glycerol, 0.32% Tris HCl, 0.02% DTT

常规信息

  • 别名
    • Embryonic stem cell specific homeobox protein (Nanog)
    • ENK
    • FLJ12581
    • hNanog
    • Homeobox protein NANOG
    • Homeobox transcription factor Nanog
    • homeobox transcription factor Nanog-delta 48
    • NANOG
    • Nanog homeobox
    • NANOG_HUMAN
    see all
  • 功能Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
  • 组织特异性Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
  • 序列相似性Belongs to the Nanog homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 发展阶段Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
  • 细胞定位Nucleus.
  • Information by UniProt

Recombinant Human Nanog protein 图像

  • 15% SDS-PAGE analysis of 3 µg ab109960.

Recombinant Human Nanog protein (ab109960)参考文献

ab109960 has not yet been referenced specifically in any publications.

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