重组人Myosin light chain kinase/MLCK蛋白(ab55674)
Key features and details
- Expression system: Insect cells
- Purity: > 95% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人Myosin light chain kinase/MLCK蛋白
参阅全部 Myosin light chain kinase/MLCK 蛋白酶 -
生物活性
Specific Activity: 200nm/min/mg.
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纯度
> 95 % Densitometry.
Affinity purified. -
表达系统
Insect cells -
蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
氨基酸
1425 to 1776 -
标签
GST tag N-Terminus
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相关产品
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Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab55674 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Liquid -
补充说明
ab60014 (Mouse MYL9 full length protein) can be utilized as a substrate for assessing Kinase activity
Previously labelled as Myosin light chain kinase.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- deglutamylated form
- DKFZp686I10125
- EC 2.7.11.18
see all -
功能
Calcium/calmodulin-dependent enzyme implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activty (By similarity). Implicated in the regulation of endothelial as well as vascular permeability. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. Critical participant in signaling sequences that result in fibroblast apoptosis. -
组织特异性
Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. Telokin has been found in a wide variety of adult and fetal tissues. -
序列相似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 1 fibronectin type-III domain.
Contains 9 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
翻译后修饰
MLCK is probably down-regulated by phosphorylation.
The C-terminus is deglutamylated by AGTPBP1/ CCP1, AGBL1/CCP4 and AGBL4/CCP6, leading to the formation of Myosin light chain kinase, smooth muscle, deglutamylated form. The consequences of C-terminal deglutamylation are unknown. - Information by UniProt
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab55674 被引用在 1 文献中.
- Reindl T et al. Distinct actin-tropomyosin cofilament populations drive the functional diversification of cytoskeletal myosin motor complexes. iScience 25:104484 (2022). PubMed: 35720262