重组人Myosin light chain kinase蛋白(ab152552)

概述

描述

  • 性质
    Recombinant
  • 来源
    Wheat germ
  • 氨基酸序列
    • Accession
    • 种属
      Human
    • 序列
      MDFRANLQRQVKPKTVSEEERKVHSPQQVDFRSVLAKKGTSKTPVPEKVP PPKPATPDFRSVLGGKKKLPAENGSSSAETLNAKAVESSKPLSNAQPSGP LKPVGNAKPAETLKPMGNAKPAETLKPMGNAKPDENLKSASKEELKKDVK NDVNCKRGHAGTTDNEKRSESQGTAPAFKQKLQDVHVAEGKKLLLQCQVS SDPPATIIWTLNGKTLKTTKFIILSQEGSLCSVSIEKALPEDRGLYKCVA KNDAGQAECSCQVTVDDAPASENTKAPEMKSRRPKSSLPPVLGTESDATV KKKPAPKTPPKAAMPPQIIQFPEDQKVRAGESVELFGKVTGTQPITCTWM KFRKQIQESEHMKVENSENGSKLTILAARQEHCGCYTLLVENKLGSRQAQ VNLTVVDKPDPPAGTPCASDIRSSSLTLSWYGSSYDGGSAVQSYSIEIWD SANKTWKELATCRSTSFNVQDLLPDHEYKFRVRAINVYGTSEPSQESELT TVGEKPEEPKDEVEVSDDDEKEPEVDYRTVTINTEQKVSDFYDIEERLGS GKFGQVFRLVEKKTRKVWAGKFFKAYSAKEKENIRQEISIMNCLHHPKLV QCVDAFEEKANIVMVLEIVSGGELFERIIDEDFELTERECIKYMRQISEG VEYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGLARRLENAGSLKVLF GTPEFVAPEVINYEPIGYATDMWSIGVICYILVSGLSPFMGDNDNETLAN VTSATWDFDDEAFDEISDDAKDFISNLLKKDMKNRLDCTQCLQHPWLMKD TKNMEAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLSSMAMISGLSGRKS STGSPTSPLNAEKLESEEDVSQAFLEAVAEEKPHVKPYFSKTIRDLEVVE GSAARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDEDGNCSLIISDV CGDDDAKYTCKAVNSLGEATCTAELIVETMEEGEGEGEEEEE
    • 分子量
      137 kDa including tags
    • 氨基酸
      1 to 992

技术指标

Our Abpromise guarantee covers the use of ab152552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    ELISA

    SDS-PAGE

    Western blot

  • 形式
    Liquid
  • 补充说明
    Protein concentration is above or equal to 0.05 µg/µl.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl

常规信息

  • 别名
    • deglutamylated form
    • DKFZp686I10125
    • EC 2.7.11.18
    • FLJ12216
    • Kinase related protein
    • Kinase-related protein
    • KRP
    • MLCK
    • MLCK1
    • MLCK108
    • MLCK210
    • MSTP083
    • MYLK
    • MYLK_HUMAN
    • MYLK1
    • Myosin light chain kinase
    • Myosin light polypeptide kinase
    • OTTHUMP00000180642
    • OTTHUMP00000180643
    • smMLCK
    • smooth muscle
    • Smooth muscle myosin light chain kinase
    • Telokin
    see all
  • 功能
    Calcium/calmodulin-dependent enzyme implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activty (By similarity). Implicated in the regulation of endothelial as well as vascular permeability. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. Critical participant in signaling sequences that result in fibroblast apoptosis.
  • 组织特异性
    Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. Telokin has been found in a wide variety of adult and fetal tissues.
  • 序列相似性
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 fibronectin type-III domain.
    Contains 9 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    MLCK is probably down-regulated by phosphorylation.
    The C-terminus is deglutamylated by AGTPBP1/ CCP1, AGBL1/CCP4 and AGBL4/CCP6, leading to the formation of Myosin light chain kinase, smooth muscle, deglutamylated form. The consequences of C-terminal deglutamylation are unknown.
  • Information by UniProt

图片

  • 12.5% SDS-PAGE analysis of ab152552 stained with Coomassie Blue.

文献

ab152552 has not yet been referenced specifically in any publications.

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