Recombinant人MYL9 protein (ab104014)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionP24844
    • 种属Human
    • 序列MGSSHHHHHH SSGLVPRGSH MSSKRAKAKT TKKRPQRATS NVFAMFDQSQ IQEFKEAFNM IDQNRDGFID KEDLHDMLAS LGKNPTDEYL EGMMSEAPGP INFTMFLTMF GEKLNGTDPE DVIRNAFACF DEEASGFIHE DHLRELLTTM GDRFTDEEVD EMYREAPIDK KGNFNYVEFT RILKHGAKDK DD
    • 分子量22 kDa
    • 氨基酸1 to 172
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab104014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Mass Spectrometry

    SDS-PAGE

    Western blot

  • 质谱法
    MALDI-TOF
  • 纯度> 90 % SDS-PAGE.
    Purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 100mM Sodium chloride, 1mM DTT, pH 8

常规信息

  • 别名
    • 20 kDa myosin light chain
    • Human 20kDa myosin light chain (MLC2) mRNA complete cds
    • LC20
    • MGC3505
    • MLC 2
    • MLC-2C
    • MLC2
    • MLY 9
    • MRLC1
    • MYL9
    • MYL9_HUMAN
    • Myosin light chain 9 regulatory
    • Myosin light polypeptide 9 regulatory
    • myosin regulatory light chain 1
    • Myosin regulatory light chain 2
    • Myosin regulatory light chain 2 smooth muscle isoform
    • Myosin regulatory light chain 9
    • Myosin regulatory light chain MRLC1
    • Myosin regulatory light polypeptide 9
    • Myosin RLC
    • Myosin vascular smooth muscle light chain 2
    • MYRL2
    • OTTHUMP00000030857
    • smooth muscle isoform
    see all
  • 功能Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
  • 组织特异性Smooth muscle tissues and in some, but not all, nonmuscle cells.
  • 序列相似性Contains 3 EF-hand domains.
  • 翻译后修饰Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.
  • Information by UniProt

Recombinant Human MYL9 protein 图像

  • 15% SDS-PAGE analysis of ab104014 (3 µg)
  • Anti-MYL9 antibody (ab64161) at 1/2000 dilution + Recombinant Human MYL9 protein (ab104014) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 4 minutes

Recombinant Human MYL9 protein (ab104014)参考文献

ab104014 has not yet been referenced specifically in any publications.

Product Wall

Application Other

Review text: This recombinant protein was successfully used as a substrate for in vitro phosphorylation at Serine 19. A phospho-specific antibody was used as a readout on a western blot.

The supplied protein was not phosphorylated at Serine 19 when received from Abcam.
Sample: Human Recombinant protein

Primary antibody (in addition to 'MYL9 protein')
Primary antibody: None used

Secondary antibody
Secondary antibody: None used

Additional data
PubMed ID: 21907820: View
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提交于 Nov 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"