Recombinant人MUC1 protein (ab80082)



  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionP15941
    • 种属Human
    • 分子量14 kDa including tags
    • 氨基酸1095 to 1140
    • 标签His tag N-Terminus
    • 额外的序列信息His tag is 4kDa, Fusion protein size is 10 kDa.


Our Abpromise guarantee covers the use of ab80082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot



  • 形式Lyophilised
  • Concentration information loading...


  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: PBS

  • 复溶Redissolve the powder with 1ml sterile water.


  • 别名
    • ADMCKD
    • ADMCKD1
    • Breast carcinoma associated antigen DF3
    • Breast carcinoma-associated antigen DF3
    • CA 15-3
    • CA15 3
    • CA15 3 antigen
    • CA15.3
    • Cancer antigen 15-3
    • Carcinoma associated mucin
    • Carcinoma-associated mucin
    • CD 227
    • CD227
    • DF3 antigen
    • EMA
    • Episialin
    • H23 antigen
    • H23AG
    • KL 6
    • KL-6
    • KL6
    • Krebs von den Lungen-6
    • MAM 6
    • MAM6
    • MCD
    • MCKD
    • MCKD1
    • Medullary cystic kidney disease 1 (autosomal dominant)
    • Medullary cystic kidney disease, autosomal dominant
    • MUC 1
    • MUC-1
    • MUC-1/SEC
    • MUC-1/X
    • MUC1
    • MUC1-alpha
    • MUC1-beta
    • MUC1-CT
    • MUC1-NT
    • MUC1/ZD
    • MUC1_HUMAN
    • Mucin 1
    • Mucin 1 transmembrane
    • Mucin 1, cell surface associated
    • Mucin-1 subunit beta
    • Peanut reactive urinary mucin
    • Peanut-reactive urinary mucin
    • PEM
    • PEMT
    • Polymorphic epithelial mucin
    • PUM
    • Tumor associated epithelial membrane antigen
    • Tumor associated epithelial mucin
    • Tumor associated mucin
    • Tumor-associated epithelial membrane antigen
    • Tumor-associated mucin
    see all
  • 功能The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.
    The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.
  • 组织特异性Expressed on the apical surface of epithelial cells, especially of airway passages, breast and uterus. Also expressed in activated and unactivated T-cells. Overexpressed in epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Isoform Y is expressed in tumor cells only.
  • 疾病相关MUC1/CA 15-3 is used as a serological clinical marker of breast cancer to monitor response to breast cancer treatment and disease recurrence (PubMed:20816948). Decreased levels over time may be indicative of a positive response to treatment. Conversely, increased levels may indicate disease progression. At an early stage disease, only 21% of patients exhibit high MUC1/CA 15-3 levels, that is why CA 15-3 is not a useful screening test. Most antibodies target the highly immunodominant core peptide domain of 20 amino acid (APDTRPAPGSTAPPAHGVTS) tandem repeats. Some antibodies recognize glycosylated epitopes.
    Medullary cystic kidney disease 1
  • 序列相似性Contains 1 SEA domain.
  • 发展阶段During fetal development, expressed at low levels in the colonic epithelium from 13 weeks of gestation.
  • 翻译后修饰Highly glycosylated (N- and O-linked carbohydrates and sialic acid). O-glycosylated to a varying degree on serine and threonine residues within each tandem repeat, ranging from mono- to penta-glycosylation. The average density ranges from about 50% in human milk to over 90% in T47D breast cancer cells. Further sialylation occurs during recycling. Membrane-shed glycoproteins from kidney and breast cancer cells have preferentially sialyated core 1 structures, while secreted forms from the same tissues display mainly core 2 structures. The O-glycosylated content is overlapping in both these tissues with terminal fucose and galactose, 2- and 3-linked galactose, 3- and 3,6-linked GalNAc-ol and 4-linked GlcNAc predominating. Differentially O-glycosylated in breast carcinomas with 3,4-linked GlcNAc. N-glycosylation consists of high-mannose, acidic complex-type and hybrid glycans in the secreted form MUC1/SEC, and neutral complex-type in the transmembrane form, MUC1/TM.
    Proteolytic cleavage in the SEA domain occurs in the endoplasmic reticulum by an autoproteolytic mechanism and requires the full-length SEA domain as well as requiring a Ser, Thr or Cys residue at the P + 1 site. Cleavage at this site also occurs on isoform MUC1/X but not on isoform MUC1/Y. Ectodomain shedding is mediated by ADAM17.
    Dual palmitoylation on cysteine residues in the CQC motif is required for recycling from endosomes back to the plasma membrane.
    Phosphorylated on tyrosines and serine residues in the C-terminal. Phosphorylation on tyrosines in the C-terminal increases the nuclear location of MUC1 and beta-catenin. Phosphorylation by PKC delta induces binding of MUC1 to beta-catenin/CTNNB1 and thus decreases the formation of the beta-catenin/E-cadherin complex. Src-mediated phosphorylation inhibits interaction with GSK3B. Src- and EGFR-mediated phosphorylation on Tyr-1229 increases binding to beta-catenin/CTNNB1. GSK3B-mediated phosphorylation on Ser-1227 decreases this interaction but restores the formation of the beta-cadherin/E-cadherin complex. On T-cell receptor activation, phosphorylated by LCK. PDGFR-mediated phosphorylation increases nuclear colocalization of MUC1CT and CTNNB1.
    The N-terminal sequence has been shown to begin at position 24 or 28.
  • 细胞定位Secreted; Cell membrane. Cytoplasm. Nucleus. On EGF and PDGFRB stimulation, transported to the nucleus through interaction with CTNNB1, a process which is stimulated by phosphorylation. On HRG stimulation, colocalizes with JUP/gamma-catenin at the nucleus and Apical cell membrane. Exclusively located in the apical domain of the plasma membrane of highly polarized epithelial cells. After endocytosis, internalized and recycled to the cell membrane. Located to microvilli and to the tips of long filopodial protusions.
  • Information by UniProt

Recombinant Human MUC1 protein 图像

  • 20% SDS PAGE showing ab80082 at approximately 10kDa.

Recombinant Human MUC1 protein (ab80082)参考文献

ab80082 has not yet been referenced specifically in any publications.

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Unfortunately, we do not offer a MUC1 fulllength protein. The MUC1 protein (ab80082) is only a recombinant fragment and all others are peptides. I am sorry for the inconvenience but I cannot recommend any of these ...

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Thank you for your inquiry and I sincerely apologize for the delay. I was in contact with the supplying lab and unfortunately we are not able to provide a custom preparation of this protein at this time. We also do not know if the MUC1 would bi...

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