Recombinant人MNK1 protein (ab125635)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • AccessionQ9BUB5
    • 种属Human
    • 分子量92 kDa including tags
    • 氨基酸1 to 465

技术指标

Our Abpromise guarantee covers the use of ab125635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性The specific activity of ab125635 was determined to be 16 - 20 nmol/min/mg.
  • 应用

    Western blot

    Functional Studies

    SDS-PAGE

  • 纯度> 85 % Densitometry.
    Purity was determined to be >85% by densitometry.
  • 形式Liquid
  • 补充说明

    ab204868 (Modified EIF2S peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • MAP kinase interacting kinase 1
    • MAP kinase interacting serine/threonine kinase 1
    • MAP kinase signal integrating kinase 1
    • MAP kinase signal-integrating kinase 1
    • MAP kinase-interacting serine/threonine-protein kinase 1
    • MAPK signal integrating kinase 1
    • MITOGEN-ACTIVATED PROTEIN KINASE-INTERACTING SERINE/THREONINE KINASE 1
    • mknk1
    • MKNK1_HUMAN
    • MNK 1
    • Mnk1
    see all
  • 功能May play a role in the response to environmental stress and cytokines. Appears to regulate transcription by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • 翻译后修饰Dual phosphorylation of Thr-250 and Thr-255 activates the kinase. Phosphorylation of Thr-385 activates the kinase.
  • 细胞定位Cytoplasm and Cytoplasm. Nucleus.
  • Information by UniProt

Recombinant human MNK1 protein 图像

  • Sample Kinase Assay showing the specific activity of ab125635 as 16 nmol/min/mg.
  • SDS-PAGE analysis of ab125635.

Recombinant human MNK1 protein (ab125635)参考文献

ab125635 has not yet been referenced specifically in any publications.

Product Wall

ab125635 activity was determined using substrate modified-EIF2S peptide.
ab51441 activity was determined using myelin basic protein.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"