Recombinant人Mitochondrial Pyruvate dehydrogenase kinase 1 protein (ab86986)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 序列MGSSHHHHHH SSGLVPRGSH MSSDSGSSPA SERGVPGQVD FYARFSPSPL SMKQFLDFGS VNACEKTSFM FLRQELPVRL ANIMKEISLL PDNLLRTPSV QLVQSWYIQS LQELLDFKDK SAEDAKAIYD FTDTVIRIRN RHNDVIPTMA QGVIEYKESF GVDPVTSQNV QYFLDRFYMS RISIRMLLNQ HSLLFGGKGK GSPSHRKHIG SINPNCNVLE VIKDGYENAR RLCDLYYINS PELELEELNA KSPGQPIQVV YVPSHLYHMV FELFKNAMRA TMEHHANRGV YPPIQVHVTL GNEDLTVKMS DRGGGVPLRK IDRLFNYMYS TAPRPRVETS RAVPLAGFGY GLPISRLYAQ YFQGDLKLYS LEGYGTDAVI YIKALSTDSI ERLPVYNKAA WKHYNTNHEA DDWCVPSREP KDMTTFRSA

技术指标

Our Abpromise guarantee covers the use of ab86986 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    Western blot

  • 纯度> 95 % SDS-PAGE.

  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 40% Glycerol, 20mM Tris HCl, 100mM Sodium chloride, 1mM Magnesium chloride, 0.5mM DTT, 0.1mM PMSF, 0.1mM EDTA, pH 7.0

常规信息

  • 别名
    • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial
    • HGNC:8809
    • Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1
    • PDH kinase 1
    • Pdk1
    • PDK1_HUMAN
    • Pyruvate dehydrogenase kinase isoform 1
    • Pyruvate dehydrogenase kinase, isoenzyme 1
    see all
  • 功能Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
  • 组织特异性Expressed predominantly in the heart.
  • 序列相似性Belongs to the PDK/BCKDK protein kinase family.
    Contains 1 histidine kinase domain.
  • 细胞定位Mitochondrion matrix.
  • Information by UniProt

Recombinant Human Mitochondrial Pyruvate dehydrogenase kinase 1 protein 图像

  • 15% SDS-PAGE showing ab86986 at approximately 48.6kDa (3µg).
  • Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody (ab73335) at 1 µg/ml + Recombinant Human TGFBI protein (ab86989) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds

Recombinant Human Mitochondrial Pyruvate dehydrogenase kinase 1 protein (ab86986)参考文献

ab86986 has not yet been referenced specifically in any publications.

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