Recombinant人Mitochondrial Pyruvate dehydrogenase kinase 1 protein (ab110359)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionQ15118
    • 种属Human
    • 氨基酸29 to 436
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab110359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Sandwich ELISA

    Functional Studies

  • 纯度> 90 % Purified via His tag.

  • 形式Liquid
  • 补充说明

     

    PDH Kinases (PDKs) deactivate PDH by reversible phosphorylation. In humans and rodents, 4 PDK's are present in an organ-dependent manner. In all cases, PDK's are physically part of the PDH complex (binding via lipoyl domain 2 of E2), and they phosphorylate the E1 subunit at 3 specific serine residues. PDH phosphatases remove the phosphorylation and restore activity of PDH.

    Product was previously marketed under the MitoSciences sub-brand.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.14% Sodium phosphate, 1.46% Sodium chloride, 30% Glycerol, 0.08% DTT, 0.02% EDTA, 0.05% Triton-X-100

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial
    • HGNC:8809
    • Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1
    • PDH kinase 1
    • Pdk1
    • PDK1_HUMAN
    • Pyruvate dehydrogenase kinase isoform 1
    • Pyruvate dehydrogenase kinase, isoenzyme 1
    see all
  • 功能Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
  • 组织特异性Expressed predominantly in the heart.
  • 序列相似性Belongs to the PDK/BCKDK protein kinase family.
    Contains 1 histidine kinase domain.
  • 细胞定位Mitochondrion matrix.
  • Information by UniProt

Recombinant human Mitochondrial Pyruvate dehydrogenase kinase 1 protein (ab110359)参考文献

ab110359 has not yet been referenced specifically in any publications.

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Thank you for your enquiry. This is an interesting question and I would be pleased to help you further.  In order to do so, I would appreciate if you could confirm the following: 1. Are you wanting to measure viability? Or metabolic activity? Or...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"