Recombinant人Melanoma gp100 protein (ab38124)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 氨基酸1 to 150

技术指标

Our Abpromise guarantee covers the use of ab38124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    ELISA

    Western blot

  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 50mM Tris acetate, 1mM EDTA, pH 7.5

常规信息

  • 别名
    • 95 kDa melanocyte specific secreted glycoprotein
    • 95 kDa melanocyte-specific secreted glycoprotein
    • D12S53E
    • gp100
    • M-beta
    • ME20
    • ME20 M/ME20 S
    • ME20-M
    • ME20-S
    • ME20M
    • ME20M/ME20S
    • ME20S
    • Melanocyte lineage specific antigen GP100
    • Melanocyte protein mel 17
    • Melanocyte protein Pmel 17
    • Melanocyte protein Pmel 17 precursor
    • Melanocytes lineage-specific antigen GP100
    • Melanoma associated ME20 antigen
    • Melanoma gp100
    • Melanoma-associated ME20 antigen
    • Melanosomal matrix protein 17
    • Melanosomal matrix protein17
    • P1
    • p100
    • p26
    • PMEL
    • PMEL 17
    • PMEL_HUMAN
    • PMEL17
    • Premelanosome protein
    • Secreted melanoma-associated ME20 antigen
    • SI
    • SIL
    • SILV
    • Silver (mouse homolog) like
    • Silver homolog
    • Silver locus protein homolog
    • Silver, mouse, homolog of
    see all
  • 功能Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.
  • 组织特异性Preferentially expressed in melanomas. Some expression was found in dysplastic nevi. Not found in normal tissues nor in carcinomas. Normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and during tumor growth.
  • 序列相似性Belongs to the PMEL/NMB family.
    Contains 1 PKD domain.
  • 结构域The RPT domain is essential for the generation of the fibrillar matrix of melanosomes.
    The lumenal domain is necessary for correct processing and trafficking to melanosomes.
  • 翻译后修饰A small amount of P1/P100 (major form) undergoes glycosylation to yield P2/P120 (minor form). P2 is cleaved by a furin-like proprotein convertase (PC) in a pH-dependent manner in a post-Golgi, prelysosomal compartment into two disulfide-linked subunits: a large lumenal subunit, M-alpha/ME20-S, and an integral membrane subunit, M-beta. Despite cleavage, only a small fraction of M-alpha is secreted, whereas most M-alpha and M-beta remain associated with each other intracellularly. M-alpha is further processed to M-alpha N and M-alpha C. M-alpha C further undergoes processing to yield M-alpha C1 and M-alpha C3 (M-alpha C2 in the case of PMEL17-is or PMEL17-ls). Formation of intralumenal fibrils in the melanosomes requires the formation of M-alpha that becomes incorporated into the fibrils. Stage II melanosomes harbor only Golgi-modified Pmel17 fragments that are derived from M-alpha and that bear sialylated O-linked oligosaccharides.
    N-glycosylated. O-glycosylated; contains sialic acid.
  • 细胞定位Secreted and Endoplasmic reticulum membrane. Golgi apparatus. Melanosome. Endosome > multivesicular body. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localizes predominantly to intralumenal vesicles (ILVs) within multivesicular bodies. Associates with ILVs found within the lumen of premelanosomes and melanosomes and particularly in compartments that serve as precursors to the striated stage II premelanosomes.
  • Information by UniProt

Recombinant Human Melanoma gp100 protein (ab38124)参考文献

ab38124 has not yet been referenced specifically in any publications.

Product Wall

Please note that ab38124 is not a full length protein but a recombinant fragment, corresponding to amino acids 1-150 of Human Melanoma gp100.

As for the stimulation assays, unfortunately we have not tested these proteins in stimulation assays...

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Thank you for your enquiry. The immunogen sequence is aa1-150 (from the first amino acid to aa150). It is the actual sequence, not a range. I hope this information will be helpful.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"